• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

DDX19A通过激活PI3K/AKT信号通路促进胃癌细胞的增殖和迁移。

DDX19A promotes gastric cancer cell proliferation and migration by activating the PI3K/AKT pathway.

作者信息

Cheng Yu, Lu Yanjie, Xue Jing, Wang Xuemei, Zhou Lili, Luo Yu, Li Yuhong

机构信息

Department of Pathology, Chengde Medical College, Chengde, Hebei Province, China.

Cancer Research Laboratory, Chengde Medical College, Chengde, Hebei Province, China.

出版信息

Cancer Cell Int. 2024 Aug 3;24(1):272. doi: 10.1186/s12935-024-03448-5.

DOI:10.1186/s12935-024-03448-5
PMID:39097730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11297674/
Abstract

BACKGROUND

DEAD-box RNA helicase 19 A (DDX19A) is overexpressed in cervical squamous cell carcinoma. However, its role in gastric cancer remains unclear. The present study aimed to explore the role and underlying mechanism of DDX19A in the development of gastric cancer.

METHODS

The expression of DDX19A in gastric cancer and paracancerous tissues was evaluated through quantitative polymerase chain reaction, western blotting, and immunohistochemical staining. The biological functions of DDX19A in gastric cancer were determined using CCK8, plate colony-forming, and Transwell migration assays. The specific mechanism of DDX19A in gastric cancer cells was studied using western blotting, RNA-binding protein immunoprecipitation, mRNA half-life detection, and nuclear and cytoplasmic RNA isolation.

RESULTS

DDX19A was highly expressed in gastric cancer and positively associated with malignant clinicopathological features and poor prognosis. Additionally, DDX19A promoted gastric cancer cell proliferation, migration, and epithelial-mesenchymal transition phenotypes. Mechanistically, DDX19A activated the PI3K/AKT pathway by upregulating phosphatidylinositol-3-kinase (PIK3CA) expression. Furthermore, DDX19A interacted with PIK3CA mRNA, stabilized it, and facilitated its export from the nucleus.

CONCLUSIONS

Our study reveals a novel mechanism whereby DDX19A promotes the proliferation and migration of gastric cancer cells by enhancing the stability and nuclear export of PIK3CA mRNA, thereby activating the PI3K/AKT pathway.

摘要

背景

DEAD盒RNA解旋酶19A（DDX19A）在宫颈鳞状细胞癌中过表达。然而，其在胃癌中的作用仍不清楚。本研究旨在探讨DDX19A在胃癌发生发展中的作用及潜在机制。

方法

通过定量聚合酶链反应、蛋白质免疫印迹法和免疫组织化学染色评估DDX19A在胃癌组织和癌旁组织中的表达。使用CCK8、平板集落形成和Transwell迁移试验确定DDX19A在胃癌中的生物学功能。采用蛋白质免疫印迹法、RNA结合蛋白免疫沉淀法、mRNA半衰期检测法以及细胞核和细胞质RNA分离法研究DDX19A在胃癌细胞中的具体机制。

结果

DDX19A在胃癌中高表达，且与恶性临床病理特征及不良预后呈正相关。此外，DDX19A促进胃癌细胞增殖、迁移以及上皮-间质转化表型。机制上，DDX19A通过上调磷脂酰肌醇-3-激酶（PIK3CA）表达激活PI3K/AKT通路。此外，DDX19A与PIK3CA mRNA相互作用，使其稳定，并促进其从细胞核输出。

结论

我们的研究揭示了一种新机制，即DDX19A通过增强PIK3CA mRNA的稳定性和细胞核输出，从而激活PI3K/AKT通路，促进胃癌细胞的增殖和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/44ddf1f625be/12935_2024_3448_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/25f559dd46a0/12935_2024_3448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/94fc4afe26c0/12935_2024_3448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/8ac2db96598e/12935_2024_3448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/1d4c883e6a2e/12935_2024_3448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/34a3785e9c66/12935_2024_3448_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/e8ddd3e7af9b/12935_2024_3448_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/44ddf1f625be/12935_2024_3448_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/25f559dd46a0/12935_2024_3448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/94fc4afe26c0/12935_2024_3448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/8ac2db96598e/12935_2024_3448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/1d4c883e6a2e/12935_2024_3448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/34a3785e9c66/12935_2024_3448_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/e8ddd3e7af9b/12935_2024_3448_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbc/11297674/44ddf1f625be/12935_2024_3448_Fig7_HTML.jpg

相似文献

1
DDX19A promotes gastric cancer cell proliferation and migration by activating the PI3K/AKT pathway.DDX19A通过激活PI3K/AKT信号通路促进胃癌细胞的增殖和迁移。
Cancer Cell Int. 2024 Aug 3;24(1):272. doi: 10.1186/s12935-024-03448-5.
2
DDX19A Promotes Metastasis of Cervical Squamous Cell Carcinoma by Inducing NOX1-Mediated ROS Production.DDX19A通过诱导NOX1介导的活性氧生成促进宫颈鳞状细胞癌转移。
Front Oncol. 2021 Apr 22;11:629974. doi: 10.3389/fonc.2021.629974. eCollection 2021.
3
Keratinocyte differentiation factor 1 enhances cervical cancer cell viability and migration by activating the PI3K/AKT pathway.角质细胞分化因子 1 通过激活 PI3K/AKT 通路增强宫颈癌癌细胞活力和迁移。
J Obstet Gynaecol. 2024 Dec;44(1):2362420. doi: 10.1080/01443615.2024.2362420. Epub 2024 Jun 12.
4
Effects of miR-202-5p silencing PIK3CA gene expression on proliferation, invasion, and epithelial-mesenchymal transition of cervical cancer SiHa cells through inhibiting PI3K/Akt/mTOR signaling pathway activation.沉默 miR-202-5p 对 PIK3CA 基因表达的影响通过抑制 PI3K/Akt/mTOR 信号通路的激活对宫颈癌 SiHa 细胞的增殖、侵袭和上皮间质转化的作用。
Mol Cell Biochem. 2021 Nov;476(11):4031-4044. doi: 10.1007/s11010-021-04211-4. Epub 2021 Jul 9.
5
MBD3 promotes epithelial-mesenchymal transition in gastric cancer cells by upregulating ACTG1 via the PI3K/AKT pathway.MBD3通过PI3K/AKT途径上调ACTG1，促进胃癌细胞的上皮-间质转化。
Biol Proced Online. 2024 Jan 5;26(1):1. doi: 10.1186/s12575-023-00228-9.
6
[Study on the Role and Mechanism of METTL3 Mediating the Up-regulation of 
m6A Modified Long Non-coding RNA THAP7-AS1 in Promoting the Occurrence of 
Lung Cancer].[METTL3介导m6A修饰的长链非编码RNA THAP7-AS1上调在促进肺癌发生中的作用及机制研究]
Zhongguo Fei Ai Za Zhi. 2024 Jan 2;26(12):919-933. doi: 10.3779/j.issn.1009-3419.2023.102.45.
7
CDK12 Promotes the Proliferation, Migration, and Angiogenesis of Gastric Carcinoma via Activating the PI3K/AKT/mTOR Signaling Pathway.CDK12 通过激活 PI3K/AKT/mTOR 信号通路促进胃癌的增殖、迁移和血管生成。
Appl Biochem Biotechnol. 2023 Nov;195(11):6913-6926. doi: 10.1007/s12010-023-04436-7. Epub 2023 Mar 23.
8
LncRNA DLEU2 silencing impedes the migration, invasion and EMT in gastric cancer cell by suppressing PI3K/AKT signaling pathway.LncRNA DLEU2 沉默通过抑制 PI3K/AKT 信号通路抑制胃癌细胞的迁移、侵袭和 EMT。
Immunopharmacol Immunotoxicol. 2022 Oct;44(5):719-731. doi: 10.1080/08923973.2022.2078727. Epub 2022 Jun 23.
9
N6-methyladenosine modification of hypoxia-inducible factor-1α regulates -associated gastric cancer the PI3K/AKT pathway.缺氧诱导因子-1α的N6-甲基腺苷修饰通过PI3K/AKT途径调控相关胃癌。
World J Gastrointest Oncol. 2024 Jul 15;16(7):3270-3283. doi: 10.4251/wjgo.v16.i7.3270.
10
Neogenin-1 Promotes Cell Proliferation, Motility, and Adhesion by Up-Regulation of Zinc Finger E-Box Binding Homeobox 1 Via Activating the Rac1/PI3K/AKT Pathway in Gastric Cancer Cells.新生蛋白-1通过激活Rac1/PI3K/AKT信号通路上调锌指E盒结合同源框1，从而促进胃癌细胞的增殖、迁移和黏附。
Cell Physiol Biochem. 2018;48(4):1457-1467. doi: 10.1159/000492255. Epub 2018 Jul 31.

引用本文的文献

1
DNA methyltransferase 3A: A prognostic biomarker and potential target for immunotherapy in gastric cancer.DNA甲基转移酶3A：胃癌中的一种预后生物标志物及免疫治疗的潜在靶点。
Medicine (Baltimore). 2025 Feb 14;104(7):e41578. doi: 10.1097/MD.0000000000041578.

本文引用的文献

1
The molecular mechanisms and therapeutic strategies of EMT in tumor progression and metastasis.肿瘤进展和转移中 EMT 的分子机制和治疗策略。
J Hematol Oncol. 2022 Sep 8;15(1):129. doi: 10.1186/s13045-022-01347-8.
2
DEAD-box helicase 56 functions as an oncogene promote cell proliferation and invasion in gastric cancer via the FOXO1/p21 Cip1/c-Myc signaling pathway.DEAD-box 解旋酶 56 通过 FOXO1/p21 Cip1/c-Myc 信号通路作为癌基因促进胃癌细胞增殖和侵袭。
Bioengineered. 2022 May;13(5):13970-13985. doi: 10.1080/21655979.2022.2084235.
3
The Role of DEAD-Box ATPases in Gene Expression and the Regulation of RNA-Protein Condensates.
DEAD-Box ATPases 在基因表达和 RNA-蛋白质凝聚物的调控中的作用。
Annu Rev Biochem. 2022 Jun 21;91:197-219. doi: 10.1146/annurev-biochem-032620-105429. Epub 2022 Mar 18.
4
Circ-ZEB1 promotes PIK3CA expression by silencing miR-199a-3p and affects the proliferation and apoptosis of hepatocellular carcinoma.环状 ZEB1 通过沉默 miR-199a-3p 促进 PIK3CA 的表达，并影响肝癌的增殖和凋亡。
Mol Cancer. 2022 Mar 11;21(1):72. doi: 10.1186/s12943-022-01529-5.
5
Knockdown of DEAD-box 51 inhibits tumor growth of esophageal squamous cell carcinoma the PI3K/AKT pathway.敲低 DEAD-box 51 抑制食管鳞癌细胞的生长 通过 PI3K/AKT 通路。
World J Gastroenterol. 2022 Jan 28;28(4):464-478. doi: 10.3748/wjg.v28.i4.464.
6
DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35.DDX10通过剪接RPL35促进结肠癌细胞的增殖和转移。
Cancer Cell Int. 2022 Feb 2;22(1):58. doi: 10.1186/s12935-022-02478-1.
7
The Roles of RNA Helicases in DNA Damage Repair and Tumorigenesis Reveal Precision Therapeutic Strategies.RNA解旋酶在DNA损伤修复和肿瘤发生中的作用揭示了精准治疗策略。
Cancer Res. 2022 Mar 1;82(5):872-884. doi: 10.1158/0008-5472.CAN-21-2187.
8
Silencing of let-7b-5p inhibits ovarian cancer cell proliferation and stemness characteristics by Asp-Glu-Ala-Asp-box helicase 19A.Let-7b-5p 的沉默通过 Asp-Glu-Ala-Asp-box 解旋酶 19A 抑制卵巢癌细胞增殖和干性特征。
Bioengineered. 2021 Dec;12(1):7666-7677. doi: 10.1080/21655979.2021.1982276.
9
DEAD-Box RNA Helicases in Cell Cycle Control and Clinical Therapy.细胞周期调控与临床治疗中的 DEAD-Box RNA 解旋酶。
Cells. 2021 Jun 18;10(6):1540. doi: 10.3390/cells10061540.
10
DDX10 promotes human lung carcinoma proliferation by U3 small nucleolar ribonucleoprotein IMP4.DDX10 通过 U3 小核仁核糖核蛋白 IMP4 促进人肺癌增殖。
Thorac Cancer. 2021 Jun;12(12):1873-1880. doi: 10.1111/1759-7714.13976. Epub 2021 May 11.