Department of Ultrasound, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei, China.
Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei, China.
Bioengineered. 2021 Dec;12(1):7666-7677. doi: 10.1080/21655979.2021.1982276.
The emergence and recurrence of ovarian cancer are associated with ovarian cancer stem cells. For cancer treatment, gene delivery of microbubbles (MB)-mediated microRNA (miRNA) is considered as a promising approach. In this study, our aim is to investigate the effects of MB-mediated let-7b-5p inhibitor on the proliferation and stemness characteristics of ovarian cancer (OVCA) cells. Let-7b-5p inhibitor mediated by MB was prepared (termed MB-let-7b-5p inhibitor), and the effects of MB-let-7b-5p inhibitor and let-7b-5p inhibitor on OVCA cell viability, proliferation and stemness characteristics were investigated. We found that MB-let-7b-5p inhibitor presented a higher transfection efficiency than let-7b-5p inhibitor alone. The inhibitory effect of MB-let-7b-5p inhibitor on OVCA cells was more significant than let-7b-5p inhibitor. Let-7b-5p targeted DEAD (Asp-Glu-Ala-Asp)-box helicase 19A (DDX19A), which was downregulated in OVCA cells. The downregulation of DDX19A reversed the inhibitory effects of MB-let-7b-5p inhibitor on OVCA cells. To sum up, we found that MB-let-7b-5p suppressed OVCA cell malignant behaviors by targeting DDX19A.
卵巢癌的发生和复发与卵巢癌干细胞有关。对于癌症治疗，微泡（MB）介导的 microRNA（miRNA）的基因传递被认为是一种很有前途的方法。在这项研究中，我们的目的是研究 MB 介导的 let-7b-5p 抑制剂对卵巢癌（OVCA）细胞增殖和干性特征的影响。制备了由 MB 介导的 let-7b-5p 抑制剂（称为 MB-let-7b-5p 抑制剂），并研究了 MB-let-7b-5p 抑制剂和 let-7b-5p 抑制剂对 OVCA 细胞活力、增殖和干性特征的影响。我们发现，MB-let-7b-5p 抑制剂的转染效率高于单独的 let-7b-5p 抑制剂。MB-let-7b-5p 抑制剂对 OVCA 细胞的抑制作用比 let-7b-5p 抑制剂更为显著。let-7b-5p 靶向 DEAD（天冬氨酸-谷氨酸-丙氨酸-天冬氨酸）盒解旋酶 19A（DDX19A），其在 OVCA 细胞中下调。DDX19A 的下调逆转了 MB-let-7b-5p 抑制剂对 OVCA 细胞的抑制作用。总之，我们发现 MB-let-7b-5p 通过靶向 DDX19A 抑制 OVCA 细胞的恶性行为。
