Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Cell Mol Biol (Noisy-le-grand). 2024 Jul 28;70(7):212-217. doi: 10.14715/cmb/2024.70.7.31.
Due to the widespread use of methamphetamine (METH) among reproductive-aged women, the effects of intrauterine exposure to METH need to be investigated, as previous studies on this topic have been limited. The goal of this study is to examine the influence of two regulatory genes (miRNA-151-3p and CACNA1C) on the intrauterine life of mice exposed to METH. Pregnant mice received doses of 2 and 5 mg/kg of METH and saline from day 10 of pregnancy until the end. Their offspring were then evaluated for miRNA-151-3p and CACNA1C gene expression levels using real-time PCR. The findings indicated that exposure to METH reduced the expression levels of both miRNA-151-3p and CACNA1C genes in offspring compared to the control group (p≤0.001). In conclusion, intrauterine exposure to METH leads to a decrease in expression levels of both miRNA-151-3p and CACNA1C genes, potentially disrupting regulatory pathways involving these genes and having an impact on male reproductive health.
由于在生育年龄的女性中广泛使用甲基苯丙胺（METH），需要研究宫内暴露于 METH 的影响，因为之前关于这个主题的研究有限。本研究的目的是研究两种调节基因（miRNA-151-3p 和 CACNA1C）对暴露于 METH 的小鼠宫内生活的影响。从妊娠第 10 天到妊娠结束，给怀孕的老鼠注射 2 和 5 毫克/公斤的 METH 和生理盐水。然后使用实时 PCR 评估后代中 miRNA-151-3p 和 CACNA1C 基因的表达水平。结果表明，与对照组相比，METH 暴露使后代中 miRNA-151-3p 和 CACNA1C 基因的表达水平降低（p≤0.001）。总之，宫内暴露于 METH 会导致 miRNA-151-3p 和 CACNA1C 基因的表达水平降低，可能会破坏涉及这些基因的调节途径，并对男性生殖健康产生影响。
