Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Bioelectrochemistry. 2024 Dec;160:108787. doi: 10.1016/j.bioelechem.2024.108787. Epub 2024 Aug 2.
A sandwich-type electrochemical immunosensor was proposed for the ultra-sensitive detection of CD44, a potential biomarker for breast cancer. In this design, a customized template-based ionic liquid (1-butyl-2,3-dimethylimidazolium tetrafluoroborate) carbon paste electrode (CILE) served as the sensing platform, and thionine/Au nanoparticles/covalent-organic frameworks (THI/Au/COF) were used as the signal label. Moreover, an enzyme-free signal amplification strategy was introduced by involving HO and phosphotungstate (PW) with peroxidase-like activity. Under optimized conditions, the linear range is as wide as six orders of magnitude, and the detection limit is as low as 0.71 pg mL (estimated based on S/N = 3). Average recoveries range from 98.16 %-100.1 %, with a relative standard deviation (RSD) of 1.42-8.27 % in mouse serum, and from 98.44 %-99.06 %, with an RSD of 1.14-4.84 % (n = 3) in artificial saliva. Furthermore, the immunosensor exhibits excellent specificity toward CD44, good stability, and low cost, indicating great potential for application in clinical trials.
一种三明治型电化学免疫传感器被提出用于超灵敏检测 CD44，CD44 是乳腺癌的一种潜在生物标志物。在这个设计中，定制模板的离子液体（1-丁基-2,3-二甲基咪唑四氟硼酸盐）碳糊电极（CILE）作为传感平台，并且使用噻嗪/Au 纳米粒子/共价有机框架（THI/Au/COF）作为信号标签。此外，通过涉及具有过氧化物酶样活性的 HO 和磷钨酸盐（PW）引入了无酶信号放大策略。在优化条件下，线性范围宽达六个数量级，检测限低至 0.71 pg mL（基于 S/N = 3 估计）。在小鼠血清中，平均回收率范围为 98.16%-100.1%，相对标准偏差（RSD）为 1.42%-8.27%（n = 3），在人工唾液中，平均回收率范围为 98.44%-99.06%，相对标准偏差（RSD）为 1.14%-4.84%（n = 3）。此外，该免疫传感器对 CD44 具有出色的特异性、良好的稳定性和低成本，表明其在临床试验中有很大的应用潜力。
