Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran.
Talanta. 2018 Oct 1;188:701-707. doi: 10.1016/j.talanta.2018.06.044. Epub 2018 Jun 15.
The fabrication of a novel electrochemical immunosensor for rapid and precise determination of prolactin was carried out using carbon paste electrode (CPE) consist of ionic liquid (IL) and graphite. Gold nanoparticles were employed as a modifier on the surface of CPE to immobilize the prolactin antibody (anti-PRL). The immunoassay was set up by sandwiching the antigen between prolactin antibody and the polyclonal anti-human-prolactin antibody labeled with horseradish peroxidase (HRP-labeled anti-PRL) as secondary antibody, on the surface of modified CPE. The reaction between O-aminophenol (OAP) and HO which is catalyzed by labeled HRP on the sandwich immunosensor generate a signal in differential pulse voltammetry (DPV) that is used to determine the concentration of prolactin. This immunosensor provides the measurement of prolactin concentration in a linear range of 25.0-2000.0 mIU L with a detection limit 12.5 mIU L. Moreover, it is applicable in the clinical assay of prolactin due to its high sensitivity and acceptable stability.
采用由离子液体（IL）和石墨组成的碳糊电极（CPE），制备了一种用于快速、准确测定催乳素的新型电化学免疫传感器。金纳米粒子被用作 CPE 表面的修饰剂，以固定催乳素抗体（抗-PRL）。通过将抗原夹在催乳素抗体和辣根过氧化物酶标记的多克隆抗人催乳素抗体（HRP 标记的抗-PRL）之间，在修饰后的 CPE 表面上建立免疫分析。在夹心免疫传感器上，标记的 HRP 催化 O-氨基酚（OAP）和 HO 之间的反应，在差分脉冲伏安法（DPV）中产生信号，用于确定催乳素的浓度。该免疫传感器提供了在 25.0-2000.0 mIU L 的线性范围内测量催乳素浓度的方法，检测限为 12.5 mIU L。此外，由于其高灵敏度和可接受的稳定性，该免疫传感器可应用于催乳素的临床测定。
