National Centre of Excellence for Food Engineering, Sheffield Hallam University, Sheffield S1 1WB, UK.
Bimolecular Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK.
Ultrason Sonochem. 2024 Oct;109:107012. doi: 10.1016/j.ultsonch.2024.107012. Epub 2024 Aug 2.
Ultrasound-assisted extraction (UAE) was evaluated as a green procedure to produce faba beans protein isolates from faba beans. Magnetic stirring was performed as conventional extraction. A three-level five-factor Box-Behnken Design (BBD) was applied to obtain the optimal UAE conditions to concurrently maximize extraction yield and protein content. The response surface methodology (RSM) showed a quadratic curvature for extraction yield and protein. The optimal extraction conditions were determined as: Power of 123 W, solute/solvent ratio of 0.06 (1:15 g/mL), sonication time of 41 min, and total volume of 623 mL with a desirability value of 0.82. Under these conditions, the extraction yield of 19. 75 ± 0.87 % (Protein yield of 67.84 %) and protein content of 92.87 ± 0.53 % were obtained for optimum ultrasound extraction. Control samples using magnetic stirring under similar conditions without ultrasound treatment showed an extraction yield of 16.41 ± 0.02 % (Protein yield of 54.65 %) and a protein content of 89. 88 ± 0.40 %. This shows that BBD can effectively be used to optimize the extraction of proteins from faba beans using optimal extraction conditions, resulting in a higher extraction yield and protein purity.
超声辅助提取 (UAE) 被评估为一种绿色工艺，可从蚕豆中生产蚕豆蛋白分离物。采用磁力搅拌作为常规提取方法。应用三水平五因素 Box-Behnken 设计 (BBD) 以获得最佳 UAE 条件，同时最大化提取产率和蛋白质含量。响应面法 (RSM) 显示提取产率和蛋白质呈二次曲率。最佳提取条件确定为：功率 123 W、固液比 0.06（1:15 g/mL）、超声时间 41 min 和总体积 623 mL，理想值为 0.82。在此条件下，最优超声提取的提取产率为 19.75±0.87%（蛋白质产率为 67.84%）和蛋白质含量为 92.87±0.53%。在类似条件下，没有超声处理的磁力搅拌对照样品的提取产率为 16.41±0.02%（蛋白质产率为 54.65%）和蛋白质含量为 89.88±0.40%。这表明 BBD 可以有效地用于优化最佳提取条件下的蚕豆蛋白提取，从而获得更高的提取产率和蛋白质纯度。
