Stomatology Department, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brazil; Tropical Medicine Institute, LIM-06, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
Stomatology Department, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brazil.
Clinics (Sao Paulo). 2024 Aug 3;79:100459. doi: 10.1016/j.clinsp.2024.100459. eCollection 2024.
Sjögren's Syndrome (SS) is a chronic inflammatory autoimmune exocrinopathy, and although, the role of metabolism in the autoimmune responses has been discussed in diseases such as lupus erythematosus, rheumatoid arthritis, psoriasis and scleroderma. There is a lack of information regarding the metabolic implications of SS. Considering that the disease affects primarily salivary glands; the aim of this study is to evaluate the metabolic changes in the salivary glands' microenvironment using a targeted metabolomics approach.
The saliva from 10 patients diagnosed with SS by the American-European consensus and 10 healthy volunteers was analyzed in an Ultra-high Performance Liquid Chromatograph Coupled Mass Spectrometry (UPLC-MS).
The results showed an increased concentration in SS of metabolites involved in oxidative stress such as lactate, alanine and malate, and amino acids involved in the growth and proliferation of T-cells, such as arginine, leucine valine and isoleucine.
These results revealed that is possible to differentiate the metabolic profile of SS and healthy individuals using a small amount of saliva, which in its turn may reflect the cellular changes observed in the microenvironments of damaged salivary glands from these patients.
干燥综合征（SS）是一种慢性炎症性自身免疫外分泌疾病，尽管代谢在红斑狼疮、类风湿关节炎、银屑病和硬皮病等疾病的自身免疫反应中的作用已被讨论，但关于 SS 的代谢影响的信息仍然缺乏。鉴于该疾病主要影响唾液腺，本研究旨在使用靶向代谢组学方法评估唾液腺微环境中的代谢变化。
使用超高效液相色谱-质谱联用仪（UPLC-MS）分析了 10 名经美国-欧洲共识诊断为 SS 的患者和 10 名健康志愿者的唾液。
结果显示，SS 患者中涉及氧化应激的代谢物，如乳酸盐、丙氨酸和苹果酸，以及涉及 T 细胞生长和增殖的氨基酸，如精氨酸、亮氨酸、缬氨酸和异亮氨酸的浓度增加。
这些结果表明，使用少量唾液就可以区分 SS 和健康个体的代谢谱，这反过来可能反映了这些患者受损唾液腺微环境中观察到的细胞变化。
