原发性干燥综合征患者唾液代谢特征分析。
Analysis of the saliva metabolic signature in patients with primary Sjögren's syndrome.
机构信息
Department of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
出版信息
PLoS One. 2022 Jun 2;17(6):e0269275. doi: 10.1371/journal.pone.0269275. eCollection 2022.
BACKGROUND
The saliva metabolome has been applied to explore disease biomarkers. In this study we characterized the metabolic profile of primary Sjögren's syndrome (pSS) patients and explored metabolomic biomarkers.
METHODS
This work presents a liquid chromatography-mass spectrometry-based metabolomic study of the saliva of 32 patients with pSS and 38 age- and sex-matched healthy adults. Potential pSS saliva metabolite biomarkers were explored using test group saliva samples (20 patients with pSS vs. 25 healthy adults) and were then verified by a cross-validation group (12 patients with pSS vs. 13 healthy adults).
RESULTS
Metabolic pathways, including tryptophan metabolism, tyrosine metabolism, carbon fixation, and aspartate and asparagine metabolism, were found to be significantly regulated and related to inflammatory injury, neurological cognitive impairment and the immune response. Phenylalanyl-alanine was discovered to have good predictive ability for pSS, with an area under the curve (AUC) of 0.87 in the testing group (validation group: AUC = 0.75).
CONCLUSION
Our study shows that salivary metabolomics is a useful strategy for differential analysis and biomarker discovery in pSS.
背景
唾液代谢组学已被应用于探索疾病生物标志物。在这项研究中，我们对原发性干燥综合征（pSS）患者的代谢特征进行了分析，并探索了代谢组学生物标志物。
方法
本研究采用基于液相色谱-质谱联用的代谢组学方法分析了 32 名 pSS 患者和 38 名年龄和性别匹配的健康成年人的唾液样本。使用实验组（20 名 pSS 患者 vs. 25 名健康成年人）的唾液样本探索潜在的 pSS 唾液代谢标志物，并通过交叉验证组（12 名 pSS 患者 vs. 13 名健康成年人）进行验证。
结果
研究发现，包括色氨酸代谢、酪氨酸代谢、碳固定以及天冬氨酸和天冬酰胺代谢在内的代谢途径发生了显著调节，与炎症损伤、神经认知障碍和免疫反应有关。苯丙氨酸-丙氨酸被发现对 pSS 具有良好的预测能力，在测试组中的曲线下面积（AUC）为 0.87（验证组：AUC = 0.75）。
结论
本研究表明唾液代谢组学是分析 pSS 差异和发现生物标志物的有效策略。
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