Institute for Anatomy and Cell Biology, Saarland University, Homburg 66421, Germany.
Institute of General and Surgical Pathology, Saarland University Medical Center, Homburg 66421, Germany.
Pathol Res Pract. 2024 Sep;261:155483. doi: 10.1016/j.prp.2024.155483. Epub 2024 Jul 27.
Canonical transient receptor potential channels play a crucial role in cancer cell proliferation. While TRPC6 subtype detection in submandibular glands and the relevance of some TRPC channels in this gland have been shown in animal models, its histological detection in human lacrimal and submandibular glands, as well as related tumors, lacks systematic study. Studying TRPC6 in humans could lead to new therapeutic options. This research aimed to immunohistochemically detect TRPC6 in human samples of physiological lacrimal and submandibular glands and of adenoid cystic carcinoma and mucoepidermoid carcinoma.
Seven fixed body donors and samples of six cancer patients were examined. The ten tissue samples collected from the submandibular and lacrimal glands were then processed into histological slides and stained with hematoxylin-eosin. Tumor samples were provided as sections. TRPC6 presence was determined by immunohistochemistry, which was performed by indirect detection with a primary TRPC6 antibody, a secondary HRP-conjugated antibody and the chromogen diaminobenzidine.
Results confirm TRPC6 expression in all ten physiological gland samples: all samples showed a immunohistochemical signal with varying intensity. No significant gender-specific differences could be observed. TRPC6 was detected in four of six submandibular adenoid cystic carcinoma and the mucoepidermoid carcinoma samples, especially in tumor cells' cytoplasma and nuclei. Excretory ducts consistently showed TRPC6. Mucous tubules, their nuclei and the nuclei of adipocytes generally showed no signal while serous acini and their nuclei showed a weak TRPC6 signal.
The discovery of TRPC6 in glandular tissue indicates a role in salivary gland function and calcium homeostasis is a basis for further research into its significance for tumor development in adenoid cystic carcinoma and mucoepidermoid carcinoma of salivary glands. TRPC6 could be used as a target for treatment of these tumors. However, the correlation between TRPC6 and submandibular and lacrimal gland diseases requires further exploration.
经典瞬时受体电位通道在癌细胞增殖中起着至关重要的作用。虽然已经在动物模型中检测到下颌下腺中的 TRPC6 亚型，并且某些 TRPC 通道在该腺体中的相关性已经得到证实，但在人类泪腺和下颌下腺及其相关肿瘤中，其组织学检测缺乏系统研究。研究人类中的 TRPC6 可能会带来新的治疗选择。本研究旨在通过免疫组织化学方法检测人类生理泪腺和下颌下腺以及腺样囊性癌和黏液表皮样癌中 TRPC6 的表达。
检查了 7 名固定尸体供体和 6 名癌症患者的样本。从下颌下腺和泪腺采集的 10 个组织样本随后被加工成组织学切片并用苏木精-伊红染色。肿瘤样本作为切片提供。通过间接检测用 TRPC6 抗体、HRP 缀合的二级抗体和显色剂二氨基联苯胺进行免疫组织化学检测来确定 TRPC6 的存在。
结果证实了所有 10 个生理腺体样本中 TRPC6 的表达：所有样本均显示出不同强度的免疫组织化学信号。未观察到明显的性别特异性差异。在 6 例下颌下腺腺样囊性癌和黏液表皮样癌样本中的 4 例中检测到 TRPC6，尤其是在肿瘤细胞的细胞质和细胞核中。排泄管始终显示 TRPC6。黏液小管、其细胞核和脂肪细胞的细胞核通常没有信号，而浆液性腺泡及其细胞核显示出较弱的 TRPC6 信号。
在腺体组织中发现 TRPC6 表明其在唾液腺功能和钙稳态中的作用是进一步研究其在唾液腺腺样囊性癌和黏液表皮样癌肿瘤发展中的意义的基础。TRPC6 可作为治疗这些肿瘤的靶点。然而，TRPC6 与下颌下腺和泪腺疾病之间的相关性需要进一步探索。
