Liu Arren, Ellis Dylan, Mhatre Apurv, Brahmankar Sumant, Seto Jong, Nielsen David R, Varman Arul M
Biological Design Program, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ, USA.
Chemical Engineering Program, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ, USA.
Curr Opin Biotechnol. 2024 Oct;89:103178. doi: 10.1016/j.copbio.2024.103178. Epub 2024 Aug 3.
Lignin valorization faces persistent biomanufacturing challenges due to the heterogeneous and toxic carbon substrates derived from lignin depolymerization. To address the heterogeneous nature of aromatic feedstocks, plant cell wall engineering and 'lignin first' pretreatment methods have recently emerged. Next, to convert the resulting aromatic substrates into value-added chemicals, diverse microbial host systems also continue to be developed. This includes microbes that (1) lack aromatic metabolism, (2) metabolize aromatics but not sugars, and (3) co-metabolize both aromatics and sugars, each system presenting unique pros and cons. Considering the intrinsic complexity of lignin-derived substrate mixtures, emerging and non-model microbes with native metabolism for aromatics appear poised to provide the greatest impacts on lignin valorization via biomanufacturing.
由于木质素解聚产生的异质且有毒的碳底物，木质素增值面临着持续的生物制造挑战。为了解决芳香族原料的异质性，植物细胞壁工程和“先木质素”预处理方法最近应运而生。接下来，为了将生成的芳香族底物转化为增值化学品，各种微生物宿主系统也在不断开发中。这包括以下几类微生物：（1）缺乏芳香族代谢能力的微生物；（2）能代谢芳香族化合物但不能代谢糖类的微生物；（3）能共同代谢芳香族化合物和糖类的微生物，每个系统都有其独特的优缺点。考虑到木质素衍生底物混合物的内在复杂性，具有天然芳香族代谢能力的新兴和非模式微生物似乎有望通过生物制造对木质素增值产生最大影响。
