Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.
Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China; Institute of Quality Standard and Testing Technology for Agro-products, Shandong Academy of Agricultural Sciences, Ji'nan, Shandong, China.
Sci Total Environ. 2024 Nov 10;950:175262. doi: 10.1016/j.scitotenv.2024.175262. Epub 2024 Aug 3.
The utilization of nitenpyram for aphid and whitefly control may induce environmental contamination and negative repercussions on non-target organisms. Formerly, we found that nitenpyram would pollute the peripheral and sub-peripheral areas of the adjacent mulberry orchard. Under acute toxicity conditions, nitenpyram induced oxidative damage in silkworms, affected biological metabolism, synthesis, immunity, and signal transduction. Considering the impact of nitenpyram mist drift on mulberry leaves, we investigated the effects of low concentrations of nitenpyram on silkworms. The results showed that silkworms exposed to 0.17 mg/L, 0.35 mg/L and 0.70 mg/L of nitenpyram (1/40 LC, 1/20 LC and 1/10 LC) showed obvious poisoning symptoms. The cocoon weight and cocoon shell weight decreased gradually with increases in the concentration, and these decreases prolonged the growth and development time of silkworms and induced the detoxification enzymes carboxylesterase (CarE) and glutathione-S-transferase (GST) to cope with the stress damage caused by nitenpyram. Exposure to low concentrations of nitenpyram downregulates genes involved in the drug metabolism-other enzymes and peroxisome pathway in silkworms. Additionally, through injection of miRNA mimics and inhibitors, we discovered that detoxifying enzyme pathway genes are influenced by bmo-miR-3382-3P, bmo-miR-3213-5P and bmo-miR-133, regulating the immune response of silkworms. This study provides an overall view of the toxicity and detoxification metabolism of nitenpyram in silkworm, and provides a reference for environmental assessment.
吡虫啉用于防治蚜虫和粉虱可能会导致环境污染，并对非靶标生物产生负面影响。此前，我们发现吡虫啉会污染相邻桑园的周边和次周边地区。在急性毒性条件下，吡虫啉会引起家蚕的氧化损伤，影响生物代谢、合成、免疫和信号转导。考虑到吡虫啉雾漂移对桑叶的影响，我们研究了低浓度吡虫啉对家蚕的影响。结果表明，家蚕暴露于 0.17mg/L、0.35mg/L 和 0.70mg/L 的吡虫啉（1/40LC、1/20LC 和 1/10LC）时表现出明显的中毒症状。随着浓度的增加，茧重和茧壳重逐渐下降，这延长了家蚕的生长发育时间，并诱导解毒酶羧酸酯酶（CarE）和谷胱甘肽-S-转移酶（GST）应对吡虫啉造成的应激损伤。低浓度吡虫啉暴露会下调家蚕中与药物代谢-其他酶和过氧化物酶体途径相关的基因。此外，通过注射 miRNA 模拟物和抑制剂，我们发现解毒酶途径基因受 bmo-miR-3382-3P、bmo-miR-3213-5P 和 bmo-miR-133 的影响，调节家蚕的免疫反应。本研究全面了解了吡虫啉在家蚕中的毒性和解毒代谢情况，为环境评估提供了参考。
