Division of Nephrology, Department of Medicine, Duke University School of Medicine, Durham, North Carolina, USA; Duke Transplant Center, Duke University School of Medicine, Durham, North Carolina, USA.
Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA.
Am J Transplant. 2024 Dec;24(12):2150-2156. doi: 10.1016/j.ajt.2024.07.010. Epub 2024 Aug 7.
Mouse kidney transplantation provides a powerful preclinical model for the study of kidney transplant alloimmunity. However, accurate measurement of graft function is difficult because of the inaccuracy of traditional surrogate markers serum creatinine and urea. We report the use of transdermal glomerular filtration rate measurement under the experimental conditions of unilateral nephrectomy and allogeneic kidney transplantation. Our findings demonstrate that transdermal glomerular filtration rate measurement is easy to perform, reproducible, and has more interexperimental consistency than serum creatinine or urea measurements. Most importantly, it significantly reduces the numbers of experimental animals required to detect subtle and yet clinically relevant differences in kidney function as often is the case in experimental murine kidney transplantation models.
小鼠肾移植为研究肾移植同种异体免疫提供了强有力的临床前模型。然而，由于传统替代标志物血清肌酐和尿素的不准确性，准确测量移植物功能较为困难。我们报告了在单侧肾切除和同种异体肾移植的实验条件下进行经皮肾小球滤过率测量的使用情况。我们的研究结果表明，与血清肌酐或尿素测量相比，经皮肾小球滤过率测量更容易进行、具有可重复性，并且在实验间一致性方面更有优势。最重要的是，它显著减少了检测肾功能细微但临床上相关差异所需的实验动物数量，这种情况在实验性小鼠肾移植模型中经常发生。
