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牛产肠毒素大肠杆菌与两种热稳定肠毒素基因探针的杂交

Hybridization on bovine enterotoxigenic Escherichia coli with two heat-stable enterotoxin gene probes.

作者信息

Mainil J, Bex F, Couturier M, Kaeckenbeeck A

出版信息

Am J Vet Res. 1985 Dec;46(12):2582-4.

PMID:3909866
Abstract

Hybridizations were done on bovine enterotoxigenic Escherichia coli with 2 heat-stable (ST) enterotoxin gene probes from porcine and human origin (STp and STh). Of the baby mouse-positive isolates, 56 (53%) hybridized the STp probe and 50 (47%) did not. There was no isolate that hybridized the STh probe. Hybridization with the Stp probe was more frequent (P less than 0.005) for E coli isolated from calves that died before 2 weeks of age (49 STp-positive isolates of 77 [64%] isolates) than for E coli isolated from calves that died after 2 weeks of age (2 STp-positive of 21 [10%] isolates).

摘要

使用来自猪和人的2种热稳定(ST)肠毒素基因探针(STp和STh)对牛产肠毒素大肠杆菌进行杂交试验。在对乳鼠呈阳性的分离株中,56株(53%)与STp探针杂交,50株(47%)未杂交。没有分离株与STh探针杂交。与STp探针杂交在2周龄前死亡的犊牛分离的大肠杆菌中更为频繁(P小于0.005)(77株分离株中有49株[64%]STp阳性分离株),而在2周龄后死亡的犊牛分离的大肠杆菌中则较少(21株分离株中有2株[10%]STp阳性)。

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