Swiss Tropical and Public Health Institute, Kreuzstrasse 2, 4123 Allschwil, Switzerland; University of Basel, Basel, Switzerland.
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, 11800, Malaysia.
Acta Trop. 2024 Oct;258:107336. doi: 10.1016/j.actatropica.2024.107336. Epub 2024 Aug 5.
Strongyloides stercoralis in humans often presents as a chronic asymptomatic infection. Diagnosis can be challenging due to the limited sensitivity of faecal-based parasitological techniques. A prototype lateral flow rapid diagnostic test (RDT) for the detection of specific antibodies against Strongyloides stercoralis (SsRapid) was evaluated using 143 samples from the serum bank of the Swiss Tropical and Public Health Institute. Group 1 (n = 30) comprised serum samples from larvae-positive individuals; the RDT's diagnostic sensitivity was 97 % (29/30). Group II comprised serum samples from patients with other parasitic infections (n = 86) and Swiss blood donors (n = 27); the RDT's diagnostic specificity for this group was 90 % (102/113). The RDT showed good diagnostic performance and is a promising point-of-care test for detecting human Strongyloides stercoralis infection.
人体中感染的粪类圆线虫通常表现为慢性无症状感染。由于粪便寄生虫学技术的敏感性有限，因此诊断具有挑战性。使用来自瑞士热带与公共卫生研究所血清库的 143 个样本，评估了针对粪类圆线虫（SsRapid）的特异性抗体的原型侧向流动快速诊断检测（RDT）。第 1 组（n = 30）包含幼虫阳性个体的血清样本；RDT 的诊断灵敏度为 97%（29/30）。第 2 组包含来自其他寄生虫感染患者（n = 86）和瑞士献血者（n = 27）的血清样本；该组的 RDT 诊断特异性为 90%（102/113）。该 RDT 表现出良好的诊断性能，是一种很有前途的即时检测手段，可用于检测人体粪类圆线虫感染。
