Rodpai Rutchanee, Intapan Pewpan M, Thanchomnang Tongjit, Sanpool Oranuch, Janwan Penchom, Laummaunwai Porntip, Wongkham Chaisiri, Insawang Tonkla, Maleewong Wanchai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Parasitol Res. 2016 Oct;115(10):4007-12. doi: 10.1007/s00436-016-5170-7. Epub 2016 Jun 16.
Human strongyloidiasis is a deleterious gastrointestinal disease mainly caused by Strongyloides stercoralis infection. Strongyloides stercoralis is a soil-transmitted helminthiasis that is distributed around the globe. Although definitive diagnosis is carried out through the detection of parasite objects in human stool samples, the development of reliable immunological assays is an important alternative approach for supportive diagnosis. We characterized the two sensitive and specific bands of S. stercoralis filariform larvae that reacted with human strongyloidiasis sera based on immunoblot analysis. Serum samples obtained from strongyloidiasis patients showed a sensitivity of 90 and 80 % at the approximate molecular mass of 26 and 29-kDa polypeptide bands, respectively. The reactive specificity of the 26-kDa band was 76.5 % while for the 29-kDa band was 92.2 %. Proteomic analysis identified the 26-kDa band protein was 14-3-3 protein zeta, while the 29-kDa band protein was ADP/ATP translocase 4. The results provided a basic framework for further studies regarding the potential of the S. stercoralis recombinant antigen to become a leading to diagnostic tool.
人体类圆线虫病是一种主要由粪类圆线虫感染引起的有害胃肠道疾病。粪类圆线虫是一种全球分布的土源性蠕虫病。虽然通过检测人体粪便样本中的寄生虫体进行确诊，但开发可靠的免疫测定法是辅助诊断的重要替代方法。基于免疫印迹分析，我们鉴定出了粪类圆线虫丝状蚴与人体类圆线虫病血清反应的两条敏感且特异的条带。从类圆线虫病患者获得的血清样本在大约26 kDa和29 kDa多肽条带分子量处的灵敏度分别为90%和80%。26 kDa条带的反应特异性为76.5%，而29 kDa条带的反应特异性为92.2%。蛋白质组学分析鉴定出26 kDa条带蛋白为14-3-3蛋白ζ，而29 kDa条带蛋白为ADP/ATP转位酶4。这些结果为进一步研究粪类圆线虫重组抗原成为主要诊断工具的潜力提供了基本框架。
