Caramaschi Stefania, Mangogna Alessandro, Bertoni Laura, Manfredini Marco, Farnetani Francesca, Parente Paola, Attino Vito, Cazzato Gerardo, Salviato Tiziana, Pellacani Giovanni, Reggiani Bonetti Luca
Clinical and Experimental Medicine PhD Program, Department of Biomedical, Metabolic, and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy.
Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, Modena, Italy.
Front Oncol. 2024 Jul 19;14:1336895. doi: 10.3389/fonc.2024.1336895. eCollection 2024.
Even today, melanoma is a highly aggressive neoplasm with a high mortality rate. The nodular type is very aggressive and has cerebroid nests of melanocytes (CNMs) at the growth edge, morphologically similar to the poorly differentiated neoplastic epithelial cell clusters described in colorectal, breast, and endometrioid endometrial cancers.
We selected 25 nodular melanomas (NMs) with known molecular profiles, of which the entire paraffin-embedded lesion was available. We counted CNMs under a microscopic at a magnification of 20x (i.e., a microscopic field with a major axis of 1 mm). Based on the number of CNMs in the area, melanomas were classified into three groups: G1 (CNMs ranging from 0 to 4), G2 (CNMs ranging from 5 to 9), and G3 (CNMs ≥ 10). The presence of CNMs and their counts were compared with molecular and histopathological data.
Seventeen (NMs) were grouped as G1 (68%), 5 as G2 (20%), and 3 as G3 (12%) based on CNMs count. The presence of CNMs correlated with epithelioid cell morphology ( < 0.05), Clark IV and V levels ( < 0.05), vascular invasion ( < 0.05), and biological mutants ( < 0.05). Melanomas with ≥ 10 CNMs more frequently show ulceration ( < 0.02) and the BRAF V600E mutation ( < 0.02).
CNMs count has a predictive role regardless of tumor size; their association with the BRAF V600E mutation suggests their predictive significance in response to biologics. However, further investigations are needed to strengthen this hypothesis.
即使在今天，黑色素瘤仍是一种侵袭性很强且死亡率很高的肿瘤。结节型黑色素瘤侵袭性极强，在生长边缘有脑回样黑素细胞巢（CNMs），在形态上类似于结直肠癌、乳腺癌和子宫内膜样子宫内膜癌中描述的低分化肿瘤上皮细胞簇。
我们选择了25例已知分子特征且有完整石蜡包埋病变的结节型黑色素瘤（NMs）。我们在20倍放大倍数（即长轴为1毫米的显微镜视野）下在显微镜下计数CNMs。根据该区域内CNMs的数量，黑色素瘤被分为三组：G1组（CNMs数量为0至4个）、G2组（CNMs数量为5至9个）和G3组（CNMs数量≥10个）。将CNMs的存在情况及其计数与分子和组织病理学数据进行比较。
根据CNMs计数，17例（NMs）被归为G1组（68%），5例为G2组（20%），3例为G3组（12%）。CNMs的存在与上皮样细胞形态（<0.05）、Clark IV级和V级（<0.05）、血管侵犯（<0.05）以及生物学突变（<0.05）相关。CNMs数量≥10个的黑色素瘤更常出现溃疡（<0.02）和BRAF V600E突变（<0.02）。
无论肿瘤大小，CNMs计数都具有预测作用；它们与BRAF V600E突变的关联表明其在生物制剂反应方面具有预测意义。然而，需要进一步研究来强化这一假设。
