Kondo H, Kuramoto H, Iwanaga T, Fujita T
Arch Histol Jpn. 1985 Oct;48(4):421-6. doi: 10.1679/aohc.48.421.
By means of immunohistochemistry combined with fluorescence microscopy, a cerebellar Purkinje cell-specific protein, also named spot 35 protein, was found to occur in all noradrenaline-chromaffin cells and some ganglion cells of the rat adrenal medulla. The immunoreactivity was localized in the chromaffin granules as well as the cytoplasmic matrix. The nucleus as well as the interior of the membranous organelles, were free of immunoreaction. No immunoreactivity was found in most of the intra-adrenal nerve fibers except for a few nerve fibers derived from the immunoreactive ganglion cells. Neither Schwann, satellite cells nor cortical cells were immunoreactive. It is suggested that some intracellular phenomenon possibly relating to the regulation of intracellular Ca-ion concentration is common to noradrenaline-chromaffin cells, medullary ganglion cells and cerebellar Purkinje cells.
通过免疫组织化学结合荧光显微镜检查发现，一种小脑浦肯野细胞特异性蛋白，也称为斑点35蛋白，存在于大鼠肾上腺髓质的所有去甲肾上腺素嗜铬细胞和一些神经节细胞中。免疫反应性定位于嗜铬颗粒以及细胞质基质中。细胞核以及膜性细胞器内部均无免疫反应。除了少数源自免疫反应性神经节细胞的神经纤维外，大多数肾上腺内神经纤维均无免疫反应性。雪旺细胞、卫星细胞和皮质细胞均无免疫反应性。提示去甲肾上腺素嗜铬细胞、髓质神经节细胞和小脑浦肯野细胞可能存在一些与细胞内钙离子浓度调节有关的共同细胞内现象。
