Pelto-Huikko M, Salminen T, Hervonen A
Histochemistry. 1985;82(4):377-83. doi: 10.1007/BF00494067.
The coexistence of met5- and leu5-enkephalin-like immunoreactivities with catecholamines in the rat adrenal medulla was studied with combined fluorescence microscopy and immunocytochemistry. Both met5- and leu5-enkephalin-like immunoreactivities were localized in few heavily stained adrenaline cells and in a population of nerves innervating adrenaline cells and as well as ganglion cells among the adrenaline cells. Only occasionally single noradrenaline cells exhibited light immunostaining for both enkephalins but no positive fibers could be found around the noradrenaline cells. In electron microscope the immunoreaction was seen in the granules of the adrenaline cells and in the large synaptic vesicles of the nerve terminals around the adrenaline cells. The present findings suggest that enkephalin-like immunoreactivity coexists mainly with adrenaline in rat adrenal medulla and that the enkephalin immunoreactive terminals regulate secretion of adrenaline from rat adrenal medulla.
采用荧光显微镜和免疫细胞化学相结合的方法，研究了大鼠肾上腺髓质中蛋氨酸脑啡肽-5和亮氨酸脑啡肽-5样免疫反应性与儿茶酚胺的共存情况。蛋氨酸脑啡肽-5和亮氨酸脑啡肽-5样免疫反应性均定位于少数染色较深的肾上腺素细胞以及支配肾上腺素细胞的神经群体中，同时也存在于肾上腺素细胞之间的神经节细胞中。只有偶尔单个去甲肾上腺素细胞对两种脑啡肽呈现轻度免疫染色，但在去甲肾上腺素细胞周围未发现阳性纤维。在电子显微镜下，免疫反应见于肾上腺素细胞的颗粒以及肾上腺素细胞周围神经末梢的大突触小泡中。目前的研究结果表明，脑啡肽样免疫反应性主要与大鼠肾上腺髓质中的肾上腺素共存，并且脑啡肽免疫反应性终末调节大鼠肾上腺髓质中肾上腺素的分泌。
