Bahreyni Toosi Mohammad Taghi, Azimian Hossein, Salek Roham, Tabatabaei Seyed Abbas, Forghani Mohammad Naser, Dolat Elham
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Cancer Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Med Signals Sens. 2024 Jul 10;14:17. doi: 10.4103/jmss.jmss_40_23. eCollection 2024.
Radiotherapy is one of the routine treatment strategies for breast cancer (BC) patients. Different responses of the patient to radiation due to different intrinsic radiosensitivity (RS) were induced to the researcher try to introduce a standard assay for the prediction of RS. Clonogenic assay is recognized as a gold standard method in this subject but because of some of its disadvantages, it is needed for alternative assays. In this study, two assays were evaluated for this reason in ten BC patients with different RSs.
The peripheral blood of 10 volunteers with BC was obtained, and the peripheral blood mononuclear cells were extracted. After exposed with 2 Gy, survival fraction at 2 Gy (SF2) was calculated by clonogenic assay. γ-H2AX assay was performed for all patients, and apoptosis assay was evaluated for three represented categorized patients.
RS of patients showed SF2 and categorized in three groups (high, medium, and low RS). Double-strand breaks (DSBs) were decreased in high radiosensitive patients, but the residual DSBs were clearly higher than other two groups. It is shown that the repair system in these patients is lower active than others. Apoptosis frequency in patient 4 is highly active which could induce the enhancement of her RS.
γ-H2AX and apoptosis assays could predict the intrinsic RS, but evaluation of them separately is not sufficient for this aim. It is necessary to consider all the parameters together and consideration of the combination of assays could fit a better prediction of intrinsic RS.
放射治疗是乳腺癌（BC）患者的常规治疗策略之一。由于患者内在放射敏感性（RS）不同而对放疗产生不同反应，促使研究人员尝试引入一种预测RS的标准检测方法。克隆形成试验在该领域被认为是金标准方法，但由于其一些缺点，需要替代检测方法。本研究基于此对10例具有不同RS的BC患者进行了两种检测方法的评估。
采集10例BC志愿者的外周血，提取外周血单个核细胞。经2 Gy照射后，通过克隆形成试验计算2 Gy时的存活分数（SF2）。对所有患者进行γ-H2AX检测，并对3例代表性分类患者进行凋亡检测。
患者的RS显示出SF2，并分为三组（高、中、低RS）。高放射敏感性患者的双链断裂（DSB）减少，但残余DSB明显高于其他两组。结果表明，这些患者的修复系统活性低于其他患者。患者4的凋亡频率很高，这可能导致其RS增强。
γ-H2AX和凋亡检测可以预测内在RS，但单独对它们进行评估不足以实现这一目的。有必要综合考虑所有参数，考虑检测方法的组合可能更适合对内在RS进行更好的预测。
