Serim Ibrahim, Demirel Hasan Huseyin, Zemheri-Navruz Fahriye, Ince Sinan
Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey.
Afyon Kocatepe University, Bayat Vocational School, 03200, Afyonkarahisar, Turkey.
Toxicol Res (Camb). 2024 Aug 2;13(4):tfae120. doi: 10.1093/toxres/tfae120. eCollection 2024 Aug.
Pyraclostrobin, a strobilurin-derived fungicide, causes oxidative stress and DNA damage in the organism. Taurine plays an important role in metabolic processes such as osmoregulatory, cytoprotective, and antioxidant effects. The study aimed to investigate the protective effect of taurine in Sprague Dawley male rats exposed to pyraclostrobin. The rats were separated into 6 groups and were found 8 animals in each group. Rats were given 30 mg/kg pyraclostrobin and pyraclostrobin together with three different taurine concentrations (50, 100, and 200 mg/kg) via oral gavage for 28 days. While pyraclostrobin increased biochemical parameters, lipid peroxidation, and DNA damage, it decreased glutathione levels and enzyme activities of catalase and superoxide dismutase. Pyraclostrobin increased apoptotic, proinflammatory, and mRNA expression levels, whereas antiapoptotic gene mRNA expression levels decreased in liver tissue. Additionally, pyraclostrobin caused histopathological alterations in tissues. Taurine in a dose-dependent manner reversed the changes caused by pyraclostrobin. As a result, taurine exhibited a cytoprotective effect by showing antioxidant, anti-inflammatory, and antiapoptotic activities against oxidative damage caused by pyraclostrobin.
吡唑醚菌酯是一种源于甲氧基丙烯酸酯类的杀菌剂，可导致生物体产生氧化应激和DNA损伤。牛磺酸在渗透调节、细胞保护和抗氧化等代谢过程中发挥着重要作用。本研究旨在探讨牛磺酸对暴露于吡唑醚菌酯的斯普拉格-道利雄性大鼠的保护作用。将大鼠分为6组，每组8只。通过灌胃给予大鼠30mg/kg吡唑醚菌酯以及三种不同浓度（50、100和200mg/kg）的牛磺酸，持续28天。吡唑醚菌酯会增加生化参数、脂质过氧化和DNA损伤，同时降低谷胱甘肽水平以及过氧化氢酶和超氧化物歧化酶的酶活性。吡唑醚菌酯会增加肝脏组织中的凋亡、促炎和mRNA表达水平，而抗凋亡基因的mRNA表达水平则会降低。此外，吡唑醚菌酯会导致组织发生组织病理学改变。牛磺酸以剂量依赖的方式逆转了吡唑醚菌酯所引起的变化。结果表明，牛磺酸通过对吡唑醚菌酯引起的氧化损伤表现出抗氧化、抗炎和抗凋亡活性，从而发挥细胞保护作用。
