整合微生物16S rRNA测序和非靶向代谢组学以揭示鸡盲肠微生物组和血清代谢组的性别二态性。
Integrating microbial 16S rRNA sequencing and non-targeted metabolomics to reveal sexual dimorphism of the chicken cecal microbiome and serum metabolome.
作者信息
Yang Yongxian, Zhang Fuping, Yu Xuan, Wang Liqi, Wang Zhong
机构信息
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China.
出版信息
Front Microbiol. 2024 Jul 19;15:1403166. doi: 10.3389/fmicb.2024.1403166. eCollection 2024.
BACKGROUND
The gut microbiome plays a key role in the formation of livestock and poultry traits via serum metabolites, and empirical evidence has indicated these traits are sex-linked.
METHODS
We examined 106 chickens (54 male chickens and 52 female chickens) and analyzed cecal content samples and serum samples by 16S rRNA gene sequencing and non-targeted metabolomics, respectively.
RESULTS
The cecal microbiome of female chickens was more stable and more complex than that of the male chickens. and were enriched in male chickens, while , , unclassified_Anaerovoraceae, , and norank_Muribaculaceae were enriched in female chickens. Thirty-seven differential metabolites were identified in positive mode and 13 in negative mode, showing sex differences. Sphingomyelin metabolites possessed the strongest association with cecal microbes, while 11β-hydroxytestosterone showed a negative correlation with .
CONCLUSION
These results support the role of sexual dimorphism of the cecal microbiome and metabolome and implicate specific gender factors associated with production performance in chickens.
背景
肠道微生物群通过血清代谢物在畜禽性状形成中起关键作用，且已有经验证据表明这些性状与性别有关。
方法
我们检测了106只鸡（54只公鸡和52只母鸡），并分别通过16S rRNA基因测序和非靶向代谢组学分析了盲肠内容物样本和血清样本。
结果
母鸡的盲肠微生物群比公鸡的更稳定、更复杂。[具体微生物名称1]和[具体微生物名称2]在公鸡中富集，而[具体微生物名称3]、[具体微生物名称4]、未分类的厌氧杆菌科、[具体微生物名称5]、[具体微生物名称6]和无分类地位的毛螺菌科在母鸡中富集。在正模式下鉴定出37种差异代谢物，在负模式下鉴定出13种，显示出性别差异。鞘磷脂代谢物与盲肠微生物的关联最强，而11β-羟基睾酮与[具体微生物名称7]呈负相关。
结论
这些结果支持盲肠微生物群和代谢组的性别二态性作用，并暗示了与鸡生产性能相关的特定性别因素。
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