Gofur Md Royhan, Ogawa Kazushige
Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Laboratory of Veterinary Anatomy, Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
J Adv Vet Anim Res. 2024 Jun 24;11(2):516-523. doi: 10.5455/javar.2024.k801. eCollection 2024 Jun.
The research was aimed at determining the staining patterns of Peanut agglutinin (PNA) lectin and Ulex europaeus agglutinin I (UEA-I) lectin in postnatal developing (day 1 to 8 weeks) male genital excurrent duct epithelium in mice.
Lectin staining was performed on testis and epididymis of 1-day-old and 1-, 2-, 3-, 4-, 5-, 6-, and 8-week-old ICR mice.
Rete testis epithelia were UEA-I- and PNA-negative at all postnatal ages. PNA lectin unclearly stained the epithelial apical surface in efferent ductules (ED) and ductus epididymis up to 2 weeks, while UEA-I was negative in those epithelia. By contrast, at 3 weeks of age, PNA and/or UEA-I turned to clearly label epithelia in the excurrent duct system. At 5 weeks, efferent ductules epithelium was UEA-I-negative but strongly PNA-positive; segment I was PNA-negative but faintly UEA-I positive; segment II was weakly PNA-positive but strongly UEA-I-positive; segment III was strongly-PNA- and UEA-I-positive; segment IV was weakly UEA-I-positive but strongly PNA-positive; and segment V was faintly UEA-I-positive but strongly PNA-positive. At 5 and 8 weeks (the adult), the staining patterns of PNA and UEA-I lectins were nearly identical. Moreover, an increasing staining intensity was observed in both lectins with the advancement of age until 5 weeks of age. Additionally, it was noted that, with the exception of segment I, the staining intensity of PNA gradually increased while that of UEA-I gradually decreased towards the distal section of the ductus epididymis.
Lectin PNA and UEA-I staining, in conclusion, indicated epithelial segments in the male genital duct of mice from the postnatal developing age of 3 weeks, and up until 5 weeks, their staining patterns were nearly identical to those in adults.
本研究旨在确定花生凝集素（PNA）和欧洲荆豆凝集素I（UEA-I）在出生后发育阶段（1日龄至8周龄）雄性小鼠生殖输出管上皮中的染色模式。
对1日龄以及1、2、3、4、5、6和8周龄的ICR小鼠的睾丸和附睾进行凝集素染色。
在所有出生后的年龄段，睾丸网上皮均为UEA-I和PNA阴性。在2周龄之前，PNA凝集素对输出小管（ED）和附睾管上皮的顶端表面染色不清晰，而这些上皮中的UEA-I为阴性。相比之下，在3周龄时，PNA和/或UEA-I开始清晰地标记输出管系统中的上皮。在5周龄时，输出小管上皮为UEA-I阴性但PNA强阳性；节段I为PNA阴性但UEA-I弱阳性；节段II为PNA弱阳性但UEA-I强阳性；节段III为PNA和UEA-I强阳性；节段IV为UEA-I弱阳性但PNA强阳性；节段V为UEA-I弱阳性但PNA强阳性。在5周龄和8周龄（成年期）时，PNA和UEA-I凝集素的染色模式几乎相同。此外，在5周龄之前，随着年龄的增长，两种凝集素的染色强度均增加。另外，值得注意的是，除节段I外，PNA的染色强度朝着附睾管远端逐渐增加，而UEA-I的染色强度逐渐降低。
总之，凝集素PNA和UEA-I染色表明，在出生后3周龄至5周龄的雄性小鼠生殖管道中存在上皮节段，并且它们的染色模式与成年小鼠几乎相同。
