Spencer T E, Wiley A A, Bartol F F
Department of Animal and Dairy Sciences, Auburn University, Alabama 36849-5415.
J Histochem Cytochem. 1992 Dec;40(12):1937-42. doi: 10.1177/40.12.1453010.
To identify lectin binding sites and to determine if lectin binding patterns change with age in developing neonatal porcine uterine tissues, gilts (n = 3/day) were hysterectomized on Day 0 (birth), 7, 14, 28, 42, or 56. Lectin binding was visualized in Bouin's-fixed uterine tissues with seven biotinylated lectins (ConA, DBA, PNA, RCA-I, SBA, UEA-I, and WGA) and avidin-peroxidase staining procedures. Lectin specificities were demonstrated by pre-incubating lectins with appropriate inhibitory sugars (0.2 M). Staining intensity was evaluated visually (absent, weak, moderate, or strong) for three endometrial tissues; luminal epithelium, glandular epithelium, and stroma. Staining intensities for DBA, PNA, SBA, and WGA were not affected by neonatal age. Staining with these lectins was greater in uterine epithelium (moderate or strong) than in stroma (weak). In contrast, binding patterns for ConA, UEA-I, and RCA-I were affected by neonatal age. Strong epithelial staining associated with ConA binding was observed on all days, whereas stromal ConA staining decreased in intensity from moderate to weak after Day 14. Epithelial staining with UEA-I increased from moderate to strong after Day 28, whereas stromal UEA-I staining decreased from moderate to weak after day 28. Staining with RCA-I was homogeneous for luminal epithelium and stroma but variegated for glandular epithelium on and after Day 7. These observations indicate that a variety of lectin binding sites are present in developing neonatal porcine endometrial tissues and that developmentally related alterations in the distribution and/or orientation of glycoconjugates containing alpha-D-mannose, beta-D-galactose, beta-D-acetyl-N-galactosamine, and alpha-L-fucose residues occur between birth and Day 56 as these tissues mature.
为了识别凝集素结合位点，并确定在新生仔猪子宫组织发育过程中凝集素结合模式是否随年龄变化，在第0天（出生）、7天、14天、28天、42天或56天对后备母猪（每天n = 3）进行子宫切除术。使用七种生物素化凝集素（伴刀豆球蛋白A、大豆凝集素、花生凝集素、蓖麻凝集素I、大豆凝集素、荆豆凝集素I和小麦胚凝集素）和抗生物素蛋白-过氧化物酶染色程序，在经布因氏固定的子宫组织中观察凝集素结合情况。通过将凝集素与适当的抑制糖（0.2 M）预孵育来证明凝集素的特异性。对三个子宫内膜组织（腔上皮、腺上皮和基质）的染色强度进行视觉评估（无、弱、中度或强）。大豆凝集素、花生凝集素、大豆凝集素和小麦胚凝集素的染色强度不受新生年龄的影响。这些凝集素在子宫上皮（中度或强）中的染色比在基质（弱）中更强。相比之下，伴刀豆球蛋白A、荆豆凝集素I和蓖麻凝集素I的结合模式受新生年龄的影响。在所有天数均观察到与伴刀豆球蛋白A结合相关的强上皮染色，而在第14天后，基质中伴刀豆球蛋白A的染色强度从中度降至弱。荆豆凝集素I的上皮染色在第28天后从中度增加到强，而基质中荆豆凝集素I的染色在第28天后从中度降至弱。蓖麻凝集素I在第7天及之后对腔上皮和基质的染色均匀，但对腺上皮的染色呈斑驳状。这些观察结果表明，在新生仔猪子宫内膜组织发育过程中存在多种凝集素结合位点，并且随着这些组织的成熟，在出生至第56天之间，含有α-D-甘露糖、β-D-半乳糖、β-D-乙酰氨基半乳糖和α-L-岩藻糖残基的糖缀合物的分布和/或取向发生了与发育相关的改变。
