Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
J Immunoassay Immunochem. 2024 Sep 2;45(5):467-480. doi: 10.1080/15321819.2024.2384581. Epub 2024 Aug 5.
Overall, 20-30% of all cancers are estimated to be linked to infectious agents. Polyomaviruses are oncogenic cause in rodent models, readily transform their cells, and cause chromosomal instability in animal and human cells in-vitro. Some reports have indicated the presence of JCPyV and BKPyV in some human tumors. The JCPyV and BKPyV genome encodes some transforming proteins such as LT-Ag. Thus, these viruses could cause or promote some neoplasia, such as lymphomas, pancreatic, prostate, and colorectal cancers. Colorectal cancer (CRC) is the third most common cancer in the world. Risk factors for developing CRC are associated with personal features or habits, such as age, lifestyle, and gut microbiota.
In this study, we examined the prevalence of JCPyV and BKPyV in the 23 fecal samples of CRC patients and 24 healthy samples (control group). Virus DNA was extracted by a Favorgen DNA extraction kit. The large T antigen of JCPyV and VP1 of BKPyV were investigated by optimized multiplex PCR.
One of the samples was positive for the JCPyV (4.3%), while in the samples of healthy individuals, the JCPyV was negative. Also, positive results for BKPyV PCR were obtained for five cases (21.7%) in the samples of the CRC group and one case (4.1%) in healthy individuals.
The result showed no direct correlation between tumorigenesis and polyomavirus infections in CRC development. However, the exact role of BKPyV and JCPyV is still controversial and needs further study with larger sample size.
据估计，所有癌症中有 20-30%可归因于感染因子。多瘤病毒在啮齿动物模型中是致癌原因，能够轻易转化其细胞，并导致动物和人类细胞体外的染色体不稳定。一些报告表明，一些人类肿瘤中存在 JCPyV 和 BKPyV。JCPyV 和 BKPyV 基因组编码一些转化蛋白，如 LT-Ag。因此，这些病毒可能导致或促进某些肿瘤的发生，如淋巴瘤、胰腺、前列腺和结直肠癌。结直肠癌（CRC）是世界上第三大常见癌症。CRC 的发病风险因素与个人特征或习惯有关，如年龄、生活方式和肠道微生物群。
在这项研究中，我们检测了 23 例 CRC 患者和 24 例健康对照（对照组）粪便样本中 JCPyV 和 BKPyV 的流行情况。通过 Favorgen DNA 提取试剂盒提取病毒 DNA。通过优化的多重 PCR 检测 JCPyV 的大 T 抗原和 BKPyV 的 VP1。
一个样本 JCPyV 呈阳性（4.3%），而健康个体样本 JCPyV 呈阴性。此外，CRC 组的 5 个样本（21.7%）和 1 个健康个体样本（4.1%） BKPyV PCR 呈阳性结果。
结果表明，肿瘤发生与 CRC 发展过程中的多瘤病毒感染之间没有直接相关性。然而，BKPyV 和 JCPyV 的确切作用仍存在争议，需要进一步研究，以更大的样本量进行。
