巴西南部肾移植患者尿液样本中 BK 和 JC 多瘤病毒的分子检测与特征分析。

Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in Southern Brazil.

机构信息

Virology Laboratory, Microbiology, Immunology, and Parasitology Department, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.

出版信息

J Med Virol. 2015 Mar;87(3):522-8. doi: 10.1002/jmv.24086. Epub 2014 Dec 8.

DOI:10.1002/jmv.24086

PMID:25604458

Abstract

The human polyomaviruses JC (JCPyV) and BK (BKPyV) are widespread in the human population. Following the primary infection, virus reactivation may lead to nephropathy and graft rejection in renal transplant patients. This study was carried out to access the presence of BKPyV and JCPyV DNA in urine samples collected from renal transplant patients (n = 92) and healthy individuals (n = 88) in Porto Alegre, Rio Grande do Sul. The samples were submitted to a nested PCR. A significantly higher frequency (P < 0.001) of BKPyV was found in renal transplant patients (65.2%) in comparison to the control group (32.9%). JCPyV was detected equally in both groups. Phylogenetic analysis of both BKPyV and JCPyV amplicons demonstrates the presence of the BKPyV subtypes I and II, whereas for JCPyV, four different groups are found (1, 2, 3, and 4).

摘要

人类多瘤病毒 JC（JCPyV）和 BK（BKPyV）在人群中广泛存在。原发性感染后，病毒再激活可能导致肾移植患者发生肾病和移植物排斥。本研究旨在评估波尔图阿雷格里港肾移植患者（n=92）和健康个体（n=88）尿液样本中 BKPyV 和 JCPyV DNA 的存在情况。对样本进行巢式 PCR 检测。与对照组（32.9%）相比，肾移植患者 BKPyV 的检出率显著更高（P<0.001，65.2%）。两组 JCPyV 的检出率相同。对 BKPyV 和 JCPyV 扩增子的系统进化分析表明，存在 BKPyV 亚型 I 和 II，而 JCPyV 则发现了 4 个不同的群组（1、2、3 和 4）。