School of Chemistry, University of New South Wales (UNSW) Sydney, Sydney 2052, Australia.
Cluster for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
J Mater Chem B. 2024 Aug 28;12(34):8366-8375. doi: 10.1039/d4tb01026d.
With increasing antibiotic resistance and hospital acquired microbial infections, there has been a growing interest to explore alternate antimicrobial approaches. This is particularly challenging when aiming to protect surfaces over a large area to avoid contact mediated infection transmission. Quorum sensing (QS) inhibition has emerged as an alternate antimicrobial approach overcoming evolutionary stress driven resistance observed in antibiotic treatment. However, specific surface orientation requirements and limited work on delivery of small molecule QS inhibiting compounds have limited their widespread applicability certainly when it comes to coating large surfaces. Here, we report antimicrobial nanocomposite coatings overcoming the dependence on molecular orientation of QS inhibiting dihydropyrrol-2-ones (DHP) analogues and release small molecule analogues. In a systematic study, we developed poly(styrene--butyl acrylate)/graphene oxide (GO)/DHP analogue nanocomposite antimicrobial coatings that can be easily applied to surfaces of any length scale and studied their efficacy against . The polymer nanocomposite was designed to undergo coating formation at ambient temperature. The antimicrobial coatings exhibited DHP dose dependent antimicrobial response both in the supernatant growth media with a ∼7-log reduction in cell growth and virtually a complete inhibition in cell adhesion on the surface in the best coating compared to controls. When compared, DHP-Br coatings outperformed other DHP analogues (-F and -Ph) both in limiting the cell growth in the media and cellular adhesion on the coating surface. This is the first example of nanocomposite coatings comprising QS inhibiting compounds, and their exceptional performance is expected to pave the way for further research in the field.
随着抗生素耐药性和医院获得性微生物感染的增加，人们越来越有兴趣探索替代抗菌方法。当旨在保护大面积表面以避免接触介导的感染传播时，这尤其具有挑战性。群体感应 (QS) 抑制已成为一种替代抗菌方法，可克服抗生素治疗中观察到的进化应激驱动的耐药性。然而，特定表面取向要求和小分子 QS 抑制化合物传递的有限工作限制了它们的广泛适用性，尤其是在涂覆大表面时。在这里，我们报告了抗菌纳米复合材料涂层，克服了对 QS 抑制二氢吡咯-2-酮 (DHP) 类似物分子取向的依赖，并释放了小分子类似物。在系统研究中，我们开发了聚(苯乙烯-丁基丙烯酸酯)/氧化石墨烯 (GO)/DHP 类似物纳米复合材料抗菌涂层，可轻松应用于任何长度尺度的表面，并研究了它们对 的功效。聚合物纳米复合材料的设计目的是在环境温度下进行涂层形成。抗菌涂层表现出 DHP 剂量依赖性的抗菌反应，在上清液生长培养基中，细胞生长减少了约 7 个对数级，与对照相比，在最佳涂层上几乎完全抑制了细胞在表面的黏附。相比之下，DHP-Br 涂层在限制培养基中的细胞生长和涂层表面上的细胞黏附方面优于其他 DHP 类似物 (-F 和 -Ph)。这是包含 QS 抑制化合物的纳米复合材料涂层的第一个例子，它们的优异性能有望为该领域的进一步研究铺平道路。
