聚戊二醛：一种用于将蛋白质偶联到微球以及标记细胞表面受体的新型试剂。II. 利用非磁性和磁性聚戊二醛微球的简化标记方法。

Polyglutaraldehyde: a new reagent for coupling proteins to microspheres and for labeling cell-surface receptions. II. Simplified labeling method by means of non-magnetic and magnetic polyglutaraldehyde microspheres.

作者信息

Margel S, Zisblatt S, Rembaum A

出版信息

J Immunol Methods. 1979;28(3-4):341-53. doi: 10.1016/0022-1759(79)90199-6.

Abstract

Procedures were developed for the synthesis of a new immunoreagent in form of polyglutaraldehyde (PGL) microspheres in sizes ranging from about 50 nm to 1.5 micron. Addition of fluorochromes during synthesis yielded microspheres of high fluorescence intensity. By carrying out the polymerization of glutaraldehyde in presence of iron oxide, magnetic PGL microspheres were produced. Antibody conjugates obtained by interaction of PGL microspheres with immunoglobulins were used to label human red blood cells (RBC) and lymphocytes. A simple method for the separation of magnetically labeled human RBC from unlabeled cells was demonstrated.

摘要

已开发出合成新型免疫试剂的方法，该免疫试剂为尺寸范围约50纳米至1.5微米的聚戊二醛（PGL）微球形式。合成过程中添加荧光染料可得到高荧光强度的微球。通过在氧化铁存在下进行戊二醛聚合反应，制备出磁性PGL微球。PGL微球与免疫球蛋白相互作用得到的抗体缀合物用于标记人红细胞（RBC）和淋巴细胞。展示了一种从未标记细胞中分离磁性标记人红细胞的简单方法。