聚戊二醛：一种用于将蛋白质偶联到微球以及标记细胞表面受体的新型试剂。
Polyglutaraldehyde: a new reagent for coupling proteins to microspheres and for labeling cell-surface receptors.
作者信息
Rembaum A, Margel S, Levy J
出版信息
J Immunol Methods. 1978;24(3-4):239-50. doi: 10.1016/0022-1759(78)90128-x.
Glutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hypdrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was investigated spectrophotometrically as a function of pH and temperature. The reaction of polyglutaraldehyde was found to be faster than that of the monomer. The findings led to successful labeling of human lymphocyte subpopulations.
发现在碱性水溶液中聚合的戊二醛能与低分子量胺、免疫球蛋白和血红蛋白发生反应。聚戊二醛与亲水性微球共价结合。采用分光光度法研究了蛋白质添加到聚戊二醛衍生化微球上的速率与pH值和温度的关系。结果发现聚戊二醛的反应比单体的反应更快。这些发现成功实现了对人类淋巴细胞亚群的标记。
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