Hudault S, Bewa H, Bridonneau C, Raibaud P
Can J Microbiol. 1985 Sep;31(9):832-8. doi: 10.1139/m85-155.
The antagonistic effect exerted towards Salmonella typhimurium by the flora issued from conventional chickens was studied in gnotobiotic animals. In germfree chickens and mice inoculated with S. typhimurium, the highest bacterial counts were observed in ceca, and were not significantly different in either host. The protection afforded by the inoculation of cecal flora issued from a conventional chicken was more effective when this flora was inoculated first into germfree chickens than when it was given only after inoculation with S. typhimurium. Administration of a cecal flora from a 15-day-old chick to gnotobiotic mice and chicken resulted in the inhibition of a further intestinal colonization by S. typhimurium in both hosts. Sixteen strains were isolated among the predominant populations of the fecal flora from chicken flora recipient mice. Association of 14 strains of strictly anaerobic bacteria with 2 strains of Escherichia coli and Streptococcus faecium only decreased the number of S. typhimurium in the ileum of gnotobiotic mice, but not in their cecum. Anaerobe cultures were obtained from 10(-6) and 10(-8) dilutions prepared from the fecal flora of gnotobiotic recipient mice. Antagonistic bacteria were present only in cultures from the 10(-6) dilution. Cecal concentrations of volatile fatty acids were shown not to be the sole factor implicated in the antagonistic effect against S. typhimurium.
在无菌动物中研究了传统鸡源菌群对鼠伤寒沙门氏菌的拮抗作用。在接种了鼠伤寒沙门氏菌的无菌鸡和小鼠中，盲肠中的细菌计数最高，且在两种宿主中无显著差异。将传统鸡的盲肠菌群接种到无菌鸡中，比仅在接种鼠伤寒沙门氏菌后再给予该菌群时，所提供的保护更有效。将15日龄雏鸡的盲肠菌群给予无菌小鼠和鸡，可抑制两种宿主中鼠伤寒沙门氏菌的进一步肠道定植。从接受鸡菌群的小鼠粪便菌群的优势种群中分离出16株菌株。14株严格厌氧菌与2株大肠杆菌和粪肠球菌联合，仅减少了无菌小鼠回肠中鼠伤寒沙门氏菌的数量，而盲肠中未减少。从无菌受体小鼠粪便菌群制备的10^(-6)和10^(-8)稀释液中获得厌氧菌培养物。拮抗细菌仅存在于10^(-6)稀释液的培养物中。盲肠中挥发性脂肪酸的浓度并非是对鼠伤寒沙门氏菌产生拮抗作用的唯一因素。
