Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist, Taoyuan City, 333, Taiwan.
Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University, 4-101, Koyama-Minami, Tottori, 680-8553, Japan.
World J Microbiol Biotechnol. 2024 Aug 5;40(10):290. doi: 10.1007/s11274-024-04100-0.
Strain Lactiplantibacillus plantarum D1 with bacteriocin producing ability was found in the intestine of Gambusia affinis. The bacteriocin was found to have high inhibitory activity against multiple Streptococcus species and several other Gram-positive and Gram-negative bacteria. Bacteriocin was purified from culture supernatant by ion-exchange chromatography, Sep-Pak C cartridge, and reverse-phase high-performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectral analysis determined that purified bacteriocin has a molecular mass of 2,731 Da. A partial N-terminal sequence KRKKHKXQIYNNGM was obtained from the Edman analysis. The N-terminal sequence was employed to search against a translation of the draft genome of strain D1. The translated full amino acid sequence of the mature peptide is as follows: NH- KRKKHKCQIYNNGMPTGQYRWC, which has a molecular weight of 2738 Da. A BLAST search revealed that this bacteriocin was most similar to bactofencin A but differed from it with three amino acid residues. No identical peptide or protein has been previously reported, and this peptide, termed bactofencin YH, was therefore considered to be a new bacteriocin produced by Lactiplantibacillus plantarum D1.
从食蚊鱼肠道中发现一株具有抑菌活性的植物乳杆菌 D1，该抑菌素有广谱抑菌活性，对多种链球菌及部分革兰氏阳性和革兰氏阴性菌有抑制作用。抑菌素经离子交换层析、Sep-Pak C 小柱和反相高效液相色谱（RP-HPLC）纯化。基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析确定其相对分子质量为 2731 Da。经 Edman 降解分析获得部分 N 端序列 KRKKHKXQIYNNGM。该序列与 D1 菌株的基因组草图进行比对，推测成熟肽的氨基酸序列为：NH- KRKKHKCQIYNNGMPTGQYRWC，理论分子量为 2738 Da。BLAST 搜索显示，该抑菌素与 bactofencin A 同源性最高，但有 3 个氨基酸残基不同。目前尚未检索到相同的肽或蛋白，因此该肽被命名为 bactofencin YH，为植物乳杆菌 D1 产生的新的细菌素。
