Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Chemistry and Chemical Engineering, Hainan University, Haikou 570228, PR China.
Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Chemistry and Chemical Engineering, Hainan University, Haikou 570228, PR China; State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, PR China.
Int J Biol Macromol. 2024 Oct;277(Pt 4):134479. doi: 10.1016/j.ijbiomac.2024.134479. Epub 2024 Aug 3.
Poisonous histamine is accumulated in stale meat and fermented foods. The rapid and stable detection of histamine is essential for food safety. Herein, a ratiometric fluorometric method for histamine detection was designed through in situ preparing double-stranded DNA‑copper nanoclusters (dsDNA-Cu NCs) stained with 4',6-diamidino-2-phenylindole (DAPI). dsDNA-Cu NCs with red emission were rapidly synthesized via mixing Cu, ascorbate and dsDNA at room temperature for 5 min. When DAPI was added during preparation, DAPI coordinated with the Cu element accompanied by the quenched red emission of dsDNA-Cu NCs, and DAPI bound to dsDNA together with the enhanced blue emission of DAPI. Upon adding DAPI and histamine simultaneously, the coordination of histamine with the Cu element further decreased the red emission of dsDNA-Cu NCs, and drove the movement of DAPI from the Cu element to dsDNA along with the enhanced blue emission of DAPI. Significantly, ratiometric fluorescence was insensitive to variations in instrument and environment, causing stable measurement. Meanwhile, in situ synthesis integrated probe preparation with analyte detection, reducing time consumption. Additionally, this method quantified histamine in the concentration range of 7-50 μM with a detection limit of 3.6 μM. It was applied to determining histamine in food with satisfactory accuracy and precision.
腐胺在变质肉和发酵食品中积累。快速稳定地检测组胺对于食品安全至关重要。在此，通过原位制备用 4',6-二脒基-2-苯基吲哚 (DAPI) 染色的双链 DNA-铜纳米团簇 (dsDNA-Cu NCs)，设计了一种用于组胺检测的比率荧光法。dsDNA-Cu NCs 具有红色发射，通过在室温下混合 Cu、抗坏血酸和 dsDNA 5 分钟即可快速合成。在制备过程中加入 DAPI 时，DAPI 与 Cu 元素配位伴随着 dsDNA-Cu NCs 的红色发射猝灭，而 DAPI 与 dsDNA 结合伴随着 DAPI 的蓝色发射增强。同时加入 DAPI 和组氨酸时，组氨酸与 Cu 元素的配位进一步降低了 dsDNA-Cu NCs 的红色发射，并促使 DAPI 从 Cu 元素转移到 dsDNA 上，同时伴随着 DAPI 的蓝色发射增强。值得注意的是，比率荧光对仪器和环境的变化不敏感，从而实现了稳定的测量。同时，原位合成将探针制备与分析物检测集成在一起，减少了时间消耗。此外，该方法在 7-50 μM 的浓度范围内定量检测组氨酸，检测限为 3.6 μM。它被应用于测定食品中的组氨酸，具有令人满意的准确性和精密度。
