Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Luan, 237012, China.
The First Affiliated Hospital, College of Clinical Medicine of Henan, University of Science and Technology, Luoyang, 471003, China.
BMC Plant Biol. 2024 Aug 6;24(1):748. doi: 10.1186/s12870-024-05476-9.
Lead affects photosynthesis and growth and has serious toxic effects on plants. Here, the differential expressed proteins (DEPs) in D. huoshanense were investigated under different applications of lead acetate solutions. Using label-free quantitative proteomics methods, more than 12,000 peptides and 2,449 proteins were identified. GO and KEGG functional annotations show that these differential proteins mainly participate in carbohydrate metabolism, energy metabolism, amino acid metabolism, translation, protein folding, sorting, and degradation, as well as oxidation and reduction processes. A total of 636 DEPs were identified, and lead could induce the expression of most proteins. KEGG enrichment analysis suggested that proteins involved in processes such as homologous recombination, vitamin B6 metabolism, flavonoid biosynthesis, cellular component organisation or biogenesis, and biological regulation were significantly enriched. Nearly 40 proteins are involved in DNA replication and repair, RNA synthesis, transport, and splicing. The effect of lead stress on D. huoshanense may be achieved through photosynthesis, oxidative phosphorylation, and the production of excess antioxidant substances. The expression of 9 photosynthesis-related proteins and 12 oxidative phosphorylation-related proteins was up-regulated after lead stress. Furthermore, a total of 3 SOD, 12 POD, 3 CAT, and 7 ascorbate-related metabolic enzymes were identified. Under lead stress, almost all key enzymes involved in the synthesis of antioxidant substances are up-regulated, which may facilitate the scavenging of oxygen-free radical scavenging. The expression levels of some key enzymes involved in sugar and glycoside synthesis, the phenylpropanoid synthesis pathway, and the terpene synthesis pathway also increased. More than 30 proteins involved in heavy metal transport were also identified. Expression profiling revealed a significant rise in the expression of the ABC-type multidrug resistance transporter, copper chaperone, and P-type ATPase with exposure to lead stress. Our findings lay the basis for research on the response and resistance of D. huoshanense to heavy metal stress.
铅会影响光合作用和生长，对植物有严重的毒性作用。在这里，研究了不同浓度的醋酸铅溶液对霍山石斛差异表达蛋白（DEPs）的影响。采用无标记定量蛋白质组学方法，鉴定出超过 12000 个肽段和 2449 个蛋白质。GO 和 KEGG 功能注释表明，这些差异蛋白主要参与碳水化合物代谢、能量代谢、氨基酸代谢、翻译、蛋白质折叠、分类和降解，以及氧化还原过程。共鉴定出 636 个差异表达蛋白，铅可以诱导大多数蛋白质的表达。KEGG 富集分析表明，蛋白质参与同源重组、维生素 B6 代谢、类黄酮生物合成、细胞成分组织或生物发生以及生物调节等过程显著富集。近 40 种蛋白质参与 DNA 复制和修复、RNA 合成、运输和剪接。铅胁迫对霍山石斛的影响可能是通过光合作用、氧化磷酸化和产生过量的抗氧化物质来实现的。9 种光合作用相关蛋白和 12 种氧化磷酸化相关蛋白的表达在铅胁迫后上调。此外，共鉴定出 3 种 SOD、12 种 POD、3 种 CAT 和 7 种抗坏血酸代谢相关酶。在铅胁迫下，几乎所有参与抗氧化物质合成的关键酶都被上调，这有助于清除氧自由基。参与抗氧化物质合成的一些关键酶的表达水平也增加了，如糖和糖苷合成、苯丙素合成途径和萜烯合成途径。还鉴定出 30 多种与重金属转运相关的蛋白质。表达谱分析显示，在暴露于铅胁迫时，ABC 型多药耐药转运蛋白、铜伴侣和 P 型 ATP 酶的表达显著升高。我们的研究结果为研究霍山石斛对重金属胁迫的响应和抗性奠定了基础。
