Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, 237012, Luan, China.
Endocrinology and Metabolism Center, The First Affiliated Hospital, College of Clinical Medicine of Henan, University of Science and Technology, 471003, Luoyang, China.
BMC Genom Data. 2024 Feb 21;25(1):22. doi: 10.1186/s12863-024-01205-1.
Pb stress has a negative impact on plant growth by interfering with photosynthesis and releasing reactive oxygen species, causing major risks such as heavy metal ion accumulation in the soil matrix. A proteomics experiment was conducted to determine whether protein levels of Dendrobium huoshanense changed in response to Pb stress seven to fifteen days after being sprayed with a 200 mg/L Pb (NO) solution. The proteomic data we gathered provides a model for investigations into the mechanisms underlying Dendrobium plant resistance to heavy metal stress.
A label-free quantitative proteomics approach was employed to examine the variations in protein expression levels of D. huoshanense at different times of Pb(NO) treatment. We submitted the raw data obtained from these proteomics sequencing experiments to the ProteomeXchange database with the accession number PXD047050. 63,194 mass spectra in total were compared after being imported into the Proteome Discoverer software for database search. A total of 12,402 spectral peptides were identified with a confidence level exceeding 99%, which resulted in the identification of 2,449 significantly differential proteins. These proteins can be utilized for screening, functional annotation, and enrichment analysis of differentially expressed proteins before and after heavy metal treatment experiments.
铅胁迫通过干扰光合作用和释放活性氧物种，对植物生长产生负面影响，导致土壤基质中重金属离子积累等重大风险。进行了一项蛋白质组学实验，以确定在喷洒 200mg/L Pb(NO3)2 溶液 7 至 15 天后，霍山石斛的蛋白质水平是否会因铅胁迫而发生变化。我们收集的蛋白质组学数据为研究霍山石斛植物对重金属胁迫的抗性机制提供了模型。
采用无标记定量蛋白质组学方法，研究了不同时间点 Pb(NO3)2 处理后霍山石斛蛋白质表达水平的变化。我们将这些蛋白质组学测序实验获得的原始数据提交到 ProteomeXchange 数据库，登录号为 PXD047050。将总共 63194 个质谱导入 Proteome Discoverer 软件进行数据库搜索后进行比较。鉴定出 12402 个具有置信度超过 99%的光谱肽，共鉴定出 2449 个差异表达蛋白。这些蛋白质可用于筛选、功能注释和富集分析重金属处理前后差异表达蛋白。
