Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Morgridge Institute for Research, Madison, WI, USA.
Lab Chip. 2024 Aug 20;24(17):4198-4210. doi: 10.1039/d4lc00333k.
There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes. Improved "sample sparing" assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling. This capability would advance both clinical and basic research applications. Here we report the micro blood analysis technology (μBAT), a microfluidic platform that supports multiplexed analysis of neutrophils from a single drop of blood. We demonstrate the multiplexed orthogonal capabilities of μBAT including functional assays (phagocytosis, neutrophil extracellular traps, optical metabolic imaging) and molecular assays (gene expression, cytokine secretion). Importantly we validate our microscale platform using a macroscale benchmark assay. μBAT is compatible with lancet puncture or microdraw devices, and its design facilitates rapid operations without the need for specialized equipment. μBAT offers a new method for investigating neutrophil function in populations with restricted sample amounts.
目前，我们需要在有限的样本量基础上，用更少的资源做更多的事情，提供高度多重分析。除了实现连续采样外，改进的“节省样本”检测方法将对儿科和其他稀有样本类型的研究产生广泛影响。这种能力将推进临床和基础研究应用。在这里，我们报告了微量血液分析技术（μBAT），这是一种微流控平台，支持从单个血滴中对中性粒细胞进行多重分析。我们展示了 μBAT 的多重正交功能，包括功能测定（吞噬作用、中性粒细胞细胞外陷阱、光学代谢成像）和分子测定（基因表达、细胞因子分泌）。重要的是，我们使用宏观基准测定法验证了我们的微尺度平台。μBAT 与刺血针或微量吸管装置兼容，其设计便于快速操作，而无需专用设备。μBAT 为研究样本量有限的人群中的中性粒细胞功能提供了一种新方法。
