Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
Sci Adv. 2023 Mar 29;9(13):eadf9717. doi: 10.1126/sciadv.adf9717.
We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.
我们介绍了一种经济高效、稳健的高通量兼容血浆耗竭方法，可深度分析血浆，每次运行可检测超过 1300 种蛋白质，每天通量为 60 个样本。该方法已经通过处理超过 3000 个样本得到了充分验证，在耗竭步骤的成本约为每个样本 2.5 美元，没有明显的批次效应。
