Suhaimi Nadhirah Syafiqah, Sellyei Boglárka, Cech Gábor, Székely Csaba, Borkhanuddin Muhammad Hafiz
HUN-REN Veterinary Medical Research Institute, Budapest, Hungary.
Doctoral School of Animal Biotechnology and Animal Science, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary.
Int J Parasitol Parasites Wildl. 2024 Jul 14;24:100964. doi: 10.1016/j.ijppaw.2024.100964. eCollection 2024 Aug.
During a 2-month survey in 2023 at Tasik Telabak, Terengganu, Malaysia three distinct actinospore types, namely raabeia, triactinomyxon and aurantiactinomyxon were identified in three invertebrate host species: , sp., and sp. utilizing morphometric and molecular analyses Maximum likelihood of 18S rDNA positioned the raabeia type within the clade from fish of the Order Cypriniformes, suggesting a detected actinospore has a potential life cycle development in Cypriniformes and the genus . Both triactinomyxon and aurantiactinomyxon types were described solely based on morphology and morphometrics due to preservation error preventing the acquisition of 18S rDNA sequences. The triactinomyxon type in this study exhibited distinct morphology in spore shape and dimensions, characterized by a short style and caudal processes. Conversely, the aurantiactinomyxon type described herein possesses prominent elongated pyriform polar capsules not resembling any previously known aurantiactinomyxon types. These distinctive features, along with host species and geographical location justify their classification as novel types. Histological and microscopic analyses revealed the development of pansporocysts in the intestinal epithelium of the oligochaete host. This study marks the first descriptions of actinospore stages of myxozoans in Malaysia and the initial report of actinospores infecting host species of , sp. and sp.
2023年，在马来西亚登嘉楼州的特拉巴克湖进行了为期2个月的调查，利用形态测量和分子分析，在三种无脊椎动物宿主物种（分别为[具体物种1]、[具体物种2]和[具体物种3]）中鉴定出三种不同的放射孢子类型，即拉贝亚型、三放射孢子型和橙黄放射孢子型。18S rDNA的最大似然法将拉贝亚型定位在鲤形目鱼类的[具体分类单元]分支内，这表明检测到的放射孢子在鲤形目和[具体属]中具有潜在的生命周期发育。由于保存错误导致无法获取18S rDNA序列，三放射孢子型和橙黄放射孢子型仅基于形态学和形态测量进行描述。本研究中的三放射孢子型在孢子形状和尺寸上表现出独特的形态，其特征为短柄和尾突。相反，本文描述的橙黄放射孢子型具有突出的细长梨形极囊，与任何先前已知的橙黄放射孢子型都不同。这些独特特征，连同宿主物种和地理位置，证明它们可被归类为新型。组织学和显微镜分析揭示了寡毛纲宿主肠道上皮中泛孢子囊的发育。本研究首次描述了马来西亚粘孢子虫的放射孢子阶段，也是放射孢子感染[具体物种1]、[具体物种2]和[具体物种3]宿主物种的初步报告。
