采用分子基因分型法与血清学诊断法对镰状细胞病患者进行 RH 血型定型。
Molecular genotyping versus serological diagnosis for RH blood group typing in sickle cell patients.
机构信息
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Thalassemia & Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Expert Rev Clin Immunol. 2024 Nov;20(11):1421-1426. doi: 10.1080/1744666X.2024.2388700. Epub 2024 Aug 8.
OBJECTIVES
High rate of alloimmunization in sickle cell disease (SCD) patients poses a significant challenge in finding compatible blood unit. Accurate determination of the blood group genotype of them can help reduce the alloimmunization risk. Tetra ARMS PCR is a novel method that has been utilized recently to investigate SNPs in diseases in a fast and reliable way.
METHODS
Our study included 104 SCD and sickle thalassemia (Sβ) patients referred to Baghaei-2-Hospital of Ahvaz in 2019 using a nonrandom sampling method. Blood samples were collected for serological and molecular tests. Rh genotyping was performed using Tetra ARMS PCR and compared with the serological results.
RESULTS
Based on the Tetra ARMS PCR method, out of 104 patients, 7 (6.7%) were d/d, 40 (38.5%) were D/d, 57 (54.8%) were D/D, 25 (24%) were C/C, 59 (56.7%) were C/c, 20 (19.3%) were c/c, 4 (3.8%) were E/E, 25 (24%) were E/e, and patients 75 (72.2%) were e/e. There were discrepancies in the serological and molecular results for 11 patients.
CONCLUSION
Use of Tetra ARMS PCR in combination with serological methods for determining the Rh blood group system in donors and transfusion-dependent patients represents a remarkable transformation in the field of immunohematology.
目的
镰状细胞病（SCD）患者的高免疫率对寻找相容的血液单位构成了重大挑战。准确确定他们的血型基因型有助于降低免疫风险。四聚体 ARMS PCR 是一种最近用于快速可靠地研究疾病中 SNP 的新方法。
方法
我们的研究包括 2019 年采用非随机抽样方法转诊到阿瓦兹 Baghaei-2 医院的 104 名 SCD 和镰状细胞β贫血（Sβ）患者。采集血样进行血清学和分子检测。采用 Tetra ARMS PCR 进行 Rh 基因分型，并与血清学结果进行比较。
结果
根据 Tetra ARMS PCR 方法，在 104 例患者中，7 例（6.7%）为 d/d，40 例（38.5%）为 D/d，57 例（54.8%）为 D/D，25 例（24%）为 C/C，59 例（56.7%）为 C/c，20 例（19.3%）为 c/c，4 例（3.8%）为 E/E，25 例（24%）为 E/e，75 例（72.2%）为 e/e。11 例患者的血清学和分子结果存在差异。
结论
在供者和依赖输血的患者中，使用 Tetra ARMS PCR 结合血清学方法确定 Rh 血型系统代表了免疫血液学领域的重大转变。
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