阴道加德纳菌与阴道上皮细胞的黏附：影响黏附的变量及甲硝唑的抑制作用
Adhesion of Gardnerella vaginalis to vaginal epithelial cells: variables affecting adhesion and inhibition by metronidazole.
作者信息
Peeters M, Piot P
出版信息
Genitourin Med. 1985 Dec;61(6):391-5. doi: 10.1136/sti.61.6.391.
Abstract
Variables affecting the adherence of Gardnerella vaginalis to human vaginal epithelial cells were examined in vitro. Adherence depended on pH, with maximum attachment occurring between pH 5 and pH 6. Preincubation of the bacteria at 56 degrees C for 30 minutes and ultraviolet irradiation resulted in a noticeable decrease in adherence. In contrast, adherence was not altered by preincubating the epithelial cells under these conditions. Periodate oxidation of the vaginal cells caused an appreciable reduction in subsequent adherence of G vaginalis. None of the 19 single carbohydrates tested inhibited adherence completely. Metronidazole at subinhibitory concentrations for G vaginalis, appreciably reduced the adhesive capacity of G vaginalis, whereas subinhibitory concentrations of ampicillin did not.
摘要
在体外研究了影响阴道加德纳菌对人阴道上皮细胞黏附的变量。黏附取决于pH值，在pH 5至pH 6之间发生最大附着。将细菌在56℃预孵育30分钟和紫外线照射导致黏附显著降低。相比之下，在这些条件下对上皮细胞进行预孵育不会改变黏附。阴道细胞的高碘酸盐氧化导致随后阴道加德纳菌的黏附明显减少。所测试的19种单糖均未完全抑制黏附。对阴道加德纳菌处于亚抑制浓度的甲硝唑可显著降低阴道加德纳菌的黏附能力，而亚抑制浓度的氨苄西林则不会。
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