Scott T G, Smyth C J, Keane C T
Genitourin Med. 1987 Feb;63(1):47-53. doi: 10.1136/sti.63.1.47.
Haemagglutination and tissue culture adherence tests using a McCoy cell line were used to examine the adherence characteristics of 105 strains of Gardnerella vaginalis. Each strain represented one isolate per patient. For each patient, a direct smear of vaginal discharge was examined for clue cells. The relation between in vitro adherence and the presence of clue cells was examined. There seemed to be no appreciable relation between the presence of clue cells in smears and the haemagglutinating activity of strains. In contrast, adherence as judged by the McCoy tissue culture system showed a significant relation to the presence of clue cells (p less than 0.001). Though both adhesive characteristics were not inhibited by mannose, the mechanism of haemagglutination of human red cells appeared to differ from that of adherence of tissue culture cells. The findings imply that the clue cell phenomenon is due to attachment of adherent strains of G vaginalis to epithelial cells. Adherent strains of G vaginalis may play a part in the pathogenesis of bacterial vaginosis.
使用 McCoy 细胞系进行血凝试验和组织培养黏附试验，以检测 105 株阴道加德纳菌的黏附特性。每株代表一名患者的一个分离株。对每位患者的阴道分泌物直接涂片检查线索细胞。研究了体外黏附与线索细胞存在之间的关系。涂片中线索细胞的存在与菌株的血凝活性之间似乎没有明显关系。相比之下，通过 McCoy 组织培养系统判断的黏附与线索细胞的存在显示出显著关系（p<0.001）。虽然两种黏附特性均不受甘露糖抑制，但人红细胞的血凝机制似乎与组织培养细胞的黏附机制不同。这些发现表明，线索细胞现象是由于阴道加德纳菌黏附菌株附着于上皮细胞所致。阴道加德纳菌黏附菌株可能在细菌性阴道病的发病机制中起作用。
