Morisaki I, Kimura S, Torii M, Michalek S M, McGhee J R, Okahashi N, Hamada S
Immunobiology. 1985 Nov;170(4):293-304. doi: 10.1016/s0171-2985(85)80078-4.
Cell walls from Streptococcus pyogenes strain Sv (Group A, M type 3) were lysed with M1 endo-N-acetylmuramidase, and the group A-specific carbohydrate antigen was purified by Sephadex G-100 gel filtration. The initial eluting antigen (M1gA) peak was assessed for mitogenic and polyclonal lymphocyte-activating properties in murine spleen cell cultures. Good mitogenic responses were induced over a broad dose range (1-100 micrograms) with M1gA in both BALB/c and C3H/HeJ splenic cultures. Similar mitogenic responses were induced in nude (nu/nu) and nu/+ splenic cultures, suggesting that M1gA is a B cell mitogen. The M1gA induced anti-trinitrophenyl, anti-sheep erythrocytes, and anti-horse erythrocytes polyclonal plaque-forming cell responses in splenic cell cultures. Studies with purified splenic B cells and M1gA suggest that the mitogenic responses were indeed thymic independent. These studies clearly indicate that native group A carbohydrate antigen is a B cell mitogen and polyclonal B cell activator.
用M1内切-N-乙酰胞壁酸酶裂解化脓性链球菌菌株Sv（A组，M3型）的细胞壁，通过Sephadex G-100凝胶过滤纯化A组特异性碳水化合物抗原。在小鼠脾细胞培养物中评估最初洗脱的抗原（M1gA）峰的促有丝分裂和多克隆淋巴细胞激活特性。在BALB/c和C3H/HeJ脾细胞培养物中，M1gA在很宽的剂量范围（1-100微克）内诱导出良好的促有丝分裂反应。在裸鼠（nu/nu）和杂合子（nu/+）脾细胞培养物中诱导出类似的促有丝分裂反应，表明M1gA是一种B细胞有丝分裂原。M1gA在脾细胞培养物中诱导出抗三硝基苯、抗绵羊红细胞和抗马红细胞多克隆空斑形成细胞反应。对纯化的脾B细胞和M1gA的研究表明，促有丝分裂反应确实是不依赖胸腺的。这些研究清楚地表明，天然的A组碳水化合物抗原是一种B细胞有丝分裂原和多克隆B细胞激活剂。
