Kimura S, Hamada S, Torii M, Morisaki I, Koopman W J, Okada H, Michalek S M, McGhee J R
Scand J Immunol. 1983 Apr;17(4):313-22. doi: 10.1111/j.1365-3083.1983.tb00795.x.
A mitogenic component, designated fraction C (Fr C), has been purified from a mutanolysin enzyme digest of Actinomyces cell walls by CM Sephadex C-25 ion-exchange and G-100 gel filtration chromatography. Good mitogenic responses were obtained with Fr C over a broad dose range with peak mitogenesis seen with 500 micrograms/culture. Fraction C (mol. wt. = 35,000-40,000) consists of 75% carbohydrate and 23% protein, is non-dialysable, resistant to heat, lysozyme or protease treatment, and partially sensitive to base, and all mitogenic activity is destroyed by either periodate or acid treatment. Fraction C is a B-cell mitogen since it induced responses in nude (nu/nu) and nu/+ BALB/c spleen cell cultures and purified splenic B-cell cultures, but did not stimulate purified splenic T-cell cultures. Similar mitogenic fractions for B cells have been obtained from cell walls of A. naeslundii and from a human isolate of A. viscosus. Good polyclonal IgM synthesis and plaque-forming cell responses to hapten or erythrocytes were obtained in vitro with the purified cell wall fractions derived from all three Actinomyces strains studied. These results indicate that the Actinomyces cell wall possesses a carbohydrate-rich component which activates B cells and may represent a common determinant of this genus.
一种促有丝分裂成分，命名为C组分（Fr C），已通过CM Sephadex C - 25离子交换和G - 100凝胶过滤色谱法从放线菌细胞壁的变溶菌素酶消化物中纯化出来。Fr C在很宽的剂量范围内都能引发良好的促有丝分裂反应，在500微克/培养物时促有丝分裂作用达到峰值。C组分（分子量 = 35,000 - 40,000）由75%的碳水化合物和23%的蛋白质组成，不可透析，耐热，对溶菌酶或蛋白酶处理有抗性，对碱部分敏感，并且所有促有丝分裂活性都会被高碘酸盐或酸处理破坏。C组分是一种B细胞促有丝分裂原，因为它能在裸鼠（nu/nu）和nu/+ BALB/c脾细胞培养物以及纯化的脾B细胞培养物中诱导反应，但不会刺激纯化的脾T细胞培养物。从内氏放线菌的细胞壁以及人分离株粘性放线菌中也获得了类似的B细胞促有丝分裂组分。用所研究的所有三种放线菌菌株的纯化细胞壁组分在体外获得了良好的多克隆IgM合成以及对半抗原或红细胞的空斑形成细胞反应。这些结果表明，放线菌细胞壁具有一种富含碳水化合物的成分，可激活B细胞，可能代表了该属的一个共同决定簇。
