College of Food Science and Technology, National & Local Joint Engineering, Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Bohai University Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Jinzhou, 121013, Liaoning, China.
Mikrochim Acta. 2024 Aug 6;191(9):515. doi: 10.1007/s00604-024-06565-0.
A smartphone-assisted portable dual-mode immunoassay was constructed based on curcumin nanoparticles (CNPs) and carbon dots (CDs) for gentamicin (GEN) detection. CNPs were labeled with goat anti-mouse IgG (Ab) to create a conjugation that coupled dual signals to concentrations of GEN antigens. CNPs were introduced to pH 7.4 water and showed insignificant color and optical responses. When exposed to the high pH environment, the structure of CNPs changed and color and optical properties were restored. Because of the inner filter effect (IFE) between CNPs and CDs, the fluorescence of CNPs at 550 nm quenched the fluorescence of CDs at 450 nm. Colorimetry and ratiometric fluorescence (F/F) dual-mode immunoassay linearly correlated with GEN ranged from 10 to 10 µg/mL with a detection limit (LOD) of 8.98 × 10 µg/mL and 4.66 × 10 µg/mL, respectively. This work supplied a portable, sensitive, and specific platform to detect GEN.
基于姜黄素纳米粒子（CNPs）和碳点（CDs）构建了一种智能手机辅助的便携式双模式免疫分析方法，用于庆大霉素（GEN）检测。CNPs 被标记上羊抗鼠 IgG（Ab），以创建一种连接物，将双信号与 GEN 抗原浓度偶联。将 CNPs 引入到 pH 7.4 的水中，其颜色和光学响应没有明显变化。当暴露于高 pH 环境时，CNPs 的结构发生变化，颜色和光学性质得到恢复。由于 CNPs 和 CDs 之间的内滤效应（IFE），在 550nm 处的 CNPs 的荧光猝灭了在 450nm 处的 CDs 的荧光。比色法和比率荧光（F/F）双模式免疫分析与 GEN 的线性相关范围为 10 至 10µg/mL，检测限（LOD）分别为 8.98×10µg/mL 和 4.66×10µg/mL。这项工作提供了一种便携式、灵敏和特异的平台来检测 GEN。
