Key Laboratory of Agricultural Products Processing and Quality Control of Nanchang City/College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Guangdong Testing Institute of Product Quality Supervision, Shunde 528300, China.
Food Chem. 2023 Jan 30;400:134067. doi: 10.1016/j.foodchem.2022.134067. Epub 2022 Sep 5.
To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin Mab was prepared by hybridoma technology. Finally, a sensitive icCLEIA was developed with an 50% inhibition concentration (IC) of 0.067 ng/mL for gentamicin. The limit of detection of the icCLEIA was 0.002 ng/mL. The cross reactivity of the Mab with structural analogues were<0.01%. The recoveries of gentamicin ranged from 80 to 101% and coefficient of variation was <6.4% in pork and fish samples. Samples were detected by UPLC-MS/MS for evaluating reliability of the icCLEIA. The results suggested that the prepared anti-gentamicin Mab can be used for rapid and convenient immunoassays to detect gentamicin residues in animal tissues.
为了检测动物组织中的庆大霉素残留，制备了一种单克隆抗体（Mab），并开发了一种灵敏的间接竞争化学发光酶免疫分析（icCLEIA）方法。首先，庆大霉素与牛血清白蛋白偶联作为免疫原，用于免疫 BALB/c 小鼠。然后，通过杂交瘤技术制备抗庆大霉素 Mab。最后，建立了一种灵敏的 icCLEIA，庆大霉素的半数抑制浓度（IC）为 0.067ng/mL。icCLEIA 的检测限为 0.002ng/mL。该 Mab 与结构类似物的交叉反应性<0.01%。在猪肉和鱼肉样品中，庆大霉素的回收率为 80%至 101%，变异系数<6.4%。采用 UPLC-MS/MS 检测样品，以评估 icCLEIA 的可靠性。结果表明，所制备的抗庆大霉素 Mab 可用于快速、方便地免疫分析检测动物组织中的庆大霉素残留。
