Laboratoire de Bactériologie - Centre Hospitalier Universitaire de Besançon, Boulevard Fleming, Besançon, 25000, France.
Laboratoire associé du Centre National de Référence de la Résistance aux Antibiotiques, Centre Hospitalier Universitaire de Besançon, Besançon, 25000, France.
Eur J Clin Microbiol Infect Dis. 2024 Oct;43(10):2061-2064. doi: 10.1007/s10096-024-04895-8. Epub 2024 Aug 6.
In this study, we evaluated the performance of the EUCAST RAST method on a collection of 154 clinical strains of P. aeruginosa, including strains resistant to ceftazidime and carbapenems. While the test is convenient for routine laboratories, we observed significant rates of VME (ranging from 0.0 to 15.0%) and ME (ranging from 1.3 to 16.3%) after 6 h, particularly for key antibiotics such as ceftazidime, piperacillin/tazobactam, and meropenem. Extending the incubation time to 8 h may improve results (CA ranging from 87.2 to 99%), but caution is required in interpretation due to persistence of VME (ranging from 0.0 to 15.6%) and ME (ranging from 0.0 to 11.7%).
在这项研究中，我们评估了 EUCAST RAST 方法在 154 株临床铜绿假单胞菌菌株上的性能，包括对头孢他啶和碳青霉烯类耐药的菌株。虽然该检测方法对常规实验室来说很方便，但我们观察到在 6 小时后，VME（范围为 0.0 至 15.0%）和 ME（范围为 1.3 至 16.3%）的发生率显著升高，特别是对于头孢他啶、哌拉西林/他唑巴坦和美罗培南等关键抗生素。将孵育时间延长至 8 小时可能会改善结果（CA 范围为 87.2 至 99%），但由于 VME（范围为 0.0 至 15.6%）和 ME（范围为 0.0 至 11.7%）的持续存在，需要谨慎解释。
