Liu Chin-Feng, Young Zong-Yang, Shih Tsung-Wei, Pan Tzu-Ming, Lee Chun-Lin
Continuing Education Program of Food Biotechnology Applications, National Taitung University, Taitung, Taiwan, ROC.
Department of Life Science, National Taitung University, 369, Sec. 2, University Rd., Taitung, 95092, Taiwan, ROC.
AMB Express. 2024 Aug 6;14(1):90. doi: 10.1186/s13568-024-01735-y.
Alzheimer's disease (AD) is the most common neurodegenerative disease, with symptoms appearing in the cerebral cortex and hippocampus. amyloid β peptide (Aβ) has been shown to deposit in the brain, causing oxidative stress and inflammation, leading to impaired memory and learning. Lactocaseibacillus fermentation can produce deglycosylated isoflavones with high physiological activity, which can scavenge free radicals, enhance total antioxidant capacity and inhibit oxidative inflammatory responses. Therefore, in this study, Lactocaseibacillus paracasei subsp. paracasei NTU101 (NTU101) fermented soybean milk and its extracts were used as test substances, and AD model rats were established by infusion of Aβ40 in the brain for 28 days, and the preventive and ameliorating effects of NTU 101 fermented soymilk were discussed. Effects of soymilk and unfermented soymilk on AD, and explore its effects on AD. Main functional ingredients. The results showed that deglycosylated isoflavones in NTU101 fermented soybean milk improved AD symptoms. Mechanisms of actions include the inhibition of oxidative inflammation; reduction in the expression of risk factors for tau protein and apo E protein production, the deposition of Aβ40 around the hippocampus, and the expression of TLR-2 and RAGE proteins in astrocytes and microglia; and improvement in the memory and learning ability.
阿尔茨海默病（AD）是最常见的神经退行性疾病，症状出现在大脑皮层和海马体。淀粉样β肽（Aβ）已被证明在大脑中沉积，导致氧化应激和炎症，进而导致记忆和学习受损。乳酸干酪乳杆菌发酵可产生具有高生理活性的去糖基化异黄酮，其可清除自由基、增强总抗氧化能力并抑制氧化炎症反应。因此，在本研究中，副干酪乳杆菌副干酪亚种NTU101（NTU101）发酵豆浆及其提取物被用作受试物，通过向大脑中注入Aβ40建立AD模型大鼠28天，并探讨NTU101发酵豆浆的预防和改善作用。豆浆和未发酵豆浆对AD的影响，并探索其对AD主要功能成分的影响。结果表明，NTU101发酵豆浆中的去糖基化异黄酮改善了AD症状。作用机制包括抑制氧化炎症；降低tau蛋白和载脂蛋白E蛋白产生的危险因素的表达、海马体周围Aβ40的沉积以及星形胶质细胞和小胶质细胞中TLR-2和RAGE蛋白的表达；以及改善记忆和学习能力。
