Toktay Erdem, Selli Jale, Gurbuz Muhammed Ali, Tastan Tugba Bal, Ugan Rustem Anıl, Un Harun, Halici Zekai
Kafkas University, Faculty of Medicine, Department of Histology and Embryology, Kars.
Alanya Alaaddin Keykubat University, Faculty of Medicine, Department of Histology and Embryology, Antalya.
Iran J Basic Med Sci. 2020 Dec;23(12):1603-1609. doi: 10.22038/ijbms.2020.48294.11089.
This study aimed to examine the effects of genistein and daidzein on endometrial receptivity by histopathological, immunohistochemical, and biochemical techniques.
In this study, 72 female Sprague-Dawley rats were randomly divided into 8 groups. The endometrial receptivity model was applied to identified groups. Experimental animals were given periorally 10 mg/kg and high 40 mg/kg doses of genistein and daidzein for 5 days by gavage. At the end of the experiment, uterine tissues were evaluated histopathologically, immunohistochemically, and biochemically.
When histopathological findings were examined, significant decreases in pinopod formation were observed in high dose genistein and daidzein groups. When compared with the endometrial receptivity group, immunohistochemical staining findings showed a significant decrease in the expression of integrin β3, integrin αvβ3, LIF, and HOXA10 and an increase in MUC 1 expression in the high dose of genistein and daidzein groups. In biochemical evaluations, it was determined that genistein and daidzein increased estrogen levels and decreased progesterone levels in a dose-dependent manner.
Genistein and daidzein have a negative effect on endometrial receptivity. Therefore, individuals with a risk of infertility should pay attention to the consumption of genistein and daidzein.
本研究旨在通过组织病理学、免疫组织化学和生物化学技术研究染料木黄酮和大豆苷元对子宫内膜容受性的影响。
本研究中，72只雌性Sprague-Dawley大鼠被随机分为8组。对确定的组应用子宫内膜容受性模型。通过灌胃法给实验动物口服10mg/kg和高剂量40mg/kg的染料木黄酮和大豆苷元，持续5天。实验结束时，对子宫组织进行组织病理学、免疫组织化学和生物化学评估。
检查组织病理学结果时，在高剂量染料木黄酮和大豆苷元组中观察到胞饮突形成显著减少。与子宫内膜容受性组相比，免疫组织化学染色结果显示，高剂量染料木黄酮和大豆苷元组中整合素β3、整合素αvβ3、白血病抑制因子（LIF）和同源框A10（HOXA10）的表达显著降低，而黏蛋白1（MUC 1）表达增加。在生化评估中，确定染料木黄酮和大豆苷元以剂量依赖性方式增加雌激素水平并降低孕激素水平。
染料木黄酮和大豆苷元对子宫内膜容受性有负面影响。因此，有不孕风险的个体应注意染料木黄酮和大豆苷元的摄入。
