Henan International Laboratory for Green Pest Control/College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.
Department of Entomology and BIO5 Institute, University of Arizona, Tucson, Arizona 85721, United States.
J Agric Food Chem. 2024 Aug 21;72(33):18708-18719. doi: 10.1021/acs.jafc.4c04657. Epub 2024 Aug 6.
The extensive use of (Bt) in pest management has driven the evolution of pest resistance to Bt toxins, particularly Cry1Ac. Effective management of Bt resistance necessitates a good understanding of which pest proteins interact with Bt toxins. In this study, we screened a larval midgut cDNA library and captured 208 potential Cry1Ac-interacting proteins. Among these, we further examined the interaction between Cry1Ac and a previously unknown Cry1Ac-interacting protein, HaDALP ( death-associated LIM-only protein), as well as its role in toxicology. The results revealed that HaDALP specifically binds to both the Cry1Ac protoxin and activated toxin, significantly enhancing cell and larval tolerance to Cry1Ac. Additionally, HaDALP was overexpressed in a Cry1Ac-resistant strain. These findings reveal a greater number of Cry1Ac-interacting proteins than previously known and demonstrate, for the first time, that HaDALP reduces Cry1Ac toxicity by sequestering both the protoxin and activated toxin.
（Bt）在害虫管理中的广泛应用促使害虫对 Bt 毒素，特别是 Cry1Ac 的抗药性进化。有效管理 Bt 抗性需要很好地了解哪些害虫蛋白与 Bt 毒素相互作用。在这项研究中，我们筛选了一个幼虫中肠 cDNA 文库，捕获了 208 个潜在的 Cry1Ac 相互作用蛋白。在这些蛋白中，我们进一步研究了 Cry1Ac 与一种先前未知的 Cry1Ac 相互作用蛋白 HaDALP（死亡相关 LIM 只有蛋白）之间的相互作用，以及其在毒理学中的作用。结果表明，HaDALP 特异性地结合 Cry1Ac 原毒素和激活毒素，显著提高了细胞和幼虫对 Cry1Ac 的耐受性。此外，HaDALP 在一种 Cry1Ac 抗性品系中过表达。这些发现揭示了比以前已知的更多的 Cry1Ac 相互作用蛋白，并首次表明 HaDALP 通过隔离原毒素和激活毒素来降低 Cry1Ac 的毒性。
