比较围食膜的蛋白质组学研究为其在棉铃虫Cry1Ac 抗性中的作用提供了新的认识。
Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm .
机构信息
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing, 100193, China.
出版信息
Toxins (Basel). 2019 Feb 2;11(2):92. doi: 10.3390/toxins11020092.
Crystalline (Cry) proteins from (Bt) are widely used in sprays and transgenic crops to control insect pests, but the evolution of insect resistance threatens their long-term use. Different resistance mechanisms have been identified, but some have not been completely elucidated. Here, the transcriptome of the midgut and proteome of the peritrophic matrix (PM) were comparatively analyzed to identify potential mechanism of resistance to Cry1Ac in laboratory-selected strain XJ10 of . This strain had a 146-fold resistance to Cry1Ac protoxin and 45-fold resistance to Cry1Ac activated toxin compared with XJ strain. The mRNA and protein levels for several trypsin genes were downregulated in XJ10 compared to the susceptible strain XJ. Furthermore, 215 proteins of the PM were identified, and nearly all had corresponding mRNAs in the midgut. These results provide new insights that the PM may participate in Bt resistance.
Cry 蛋白来源于（Bt）被广泛应用于喷雾和转基因作物中以控制害虫，但昆虫抗药性的进化威胁到了它们的长期使用。已经确定了不同的抗性机制，但有些机制尚未完全阐明。在这里，对经过实验室选择的Cry1Ac 抗性菌株 XJ10 的中肠转录组和围食膜（PM）的蛋白质组进行了比较分析，以鉴定其对 Cry1Ac 的潜在抗性机制。与 XJ 菌株相比，该菌株对 Cry1Ac 原毒素的抗性提高了 146 倍，对 Cry1Ac 激活毒素的抗性提高了 45 倍。与敏感菌株 XJ 相比，XJ10 中的几种胰蛋白酶基因的 mRNA 和蛋白水平下调。此外，还鉴定出 PM 中的 215 种蛋白质，中肠中几乎都有其对应的 mRNA。这些结果提供了新的见解，即 PM 可能参与了 Bt 抗性。
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