Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
PLoS One. 2024 Aug 6;19(8):e0308132. doi: 10.1371/journal.pone.0308132. eCollection 2024.
To investigate the sex-dependent differentiation of Sox10 cells and their response to pathological conditions such as lipopolysaccharide (LPS) exposure or ischemia, we utilized Sox10 Cre-ERT2, tdTomato mice. Tamoxifen administration induced the expression of red fluorescent protein (RFP) in these cells, facilitating their subsequent tracking and analysis after LPS injection and ischemia via immunofluorescence staining. Propidium iodide (PI) was injected to label necrotic cells following LPS administration. We found that the conversion of Sox10 cells to pericytes in female mice was significantly higher than in male mice, especially in those exposed to LPS. After LPS injection, the number of PI+ necrotic cells were significantly greater in females than in males. Moreover, RFP+ cells did not co-localize with glial fibrillary acidic protein (GFAP) or cluster of differentiation 11b (CD11b). Similarly, after brain ischemia, RFP+ cells did not express cluster of differentiation 13 (CD13), neuronal nuclei (NeuN), GFAP, or ionised calcium binding adaptor molecule 1 (Iba-1). These findings indicate that the conversion of Sox10 cells to pericytes following LPS exposure is sex-dependent, with neither male nor female groups showing differentiation into other cell types after LPS exposure or under ischemic conditions. The differences in LPS-induced necrosis of pericytes between sexes may explain the variations in the conversion of Sox10 cells to pericytes in both sexes.
为了研究 Sox10 细胞的性别依赖性分化及其对脂多糖 (LPS) 暴露或缺血等病理条件的反应，我们利用 Sox10 Cre-ERT2，tdTomato 小鼠。给予他莫昔芬后，这些细胞中表达红色荧光蛋白 (RFP)，便于在 LPS 注射和缺血后通过免疫荧光染色对其进行后续追踪和分析。给予 LPS 后，通过碘化丙啶 (PI) 注射标记坏死细胞。我们发现，雌性小鼠 Sox10 细胞向周细胞的转化明显高于雄性小鼠，尤其是在 LPS 暴露的小鼠中。给予 LPS 后，雌性小鼠中 PI+坏死细胞的数量明显多于雄性小鼠。此外，RFP+细胞与神经胶质纤维酸性蛋白 (GFAP) 或分化簇 11b (CD11b) 不共定位。同样，在脑缺血后，RFP+细胞不表达分化簇 13 (CD13)、神经元核 (NeuN)、GFAP 或钙结合衔接蛋白分子 1 (Iba-1)。这些发现表明，LPS 暴露后 Sox10 细胞向周细胞的转化具有性别依赖性，无论是雄性还是雌性小鼠，在 LPS 暴露或缺血条件下均未分化为其他细胞类型。两性之间 LPS 诱导的周细胞坏死的差异可能解释了两性 Sox10 细胞向周细胞转化的差异。
