番茄枯萎病菌引起的皇冠腐烂和根部腐烂病原菌中的植物毒性异贝壳杉烯型二萜

Phytotoxic Isocassadiene-Type Diterpenoids from Tomato Fusarium Crown and Root Rot Pathogen f. sp. .

机构信息

Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, People's Republic of China.

Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.

出版信息

J Agric Food Chem. 2024 Aug 21;72(33):18478-18488. doi: 10.1021/acs.jafc.4c03345. Epub 2024 Aug 6.

DOI:10.1021/acs.jafc.4c03345

PMID:39106342

Abstract

Fusarium crown and root rot (FCRR) has emerged as a highly destructive soil-borne disease, posing a significant threat to the safe cultivation of tomatoes in recent years. The pathogen of tomato FCRR is f. sp. (). To explore potential phytotoxins from , eight undescribed diterpenoids namely fusariumic acids A-H () were isolated. Their structures were elucidated by using spectroscopic data analyses, quantum chemical calculations, and X-ray crystallography. Fusariumic acids A () and C-H () were typical isocassadiene-type diterpenoids, while fusariumic acid B () contained a cage-like structure with an unusual 7,8-seco-isocassadiene skeleton. A biosynthetic pathway of was proposed. Fusariumic acids A () and C-H () were further assessed for their phytotoxic effects on tomato seedlings at 200 μg/mL. Among them, fusariumic acid F () exhibited the strongest inhibition against the hypocotyl and root elongation of tomato seedlings, with inhibitory rates of 61.3 and 45.3%, respectively.

摘要

镰刀菌顶腐和根腐病（FCRR）是一种具有高度破坏性的土传病害，近年来对番茄的安全种植构成了重大威胁。番茄 FCRR 的病原体是 f. sp. (). 为了从中探索潜在的植物毒素，分离得到了八种未描述的二萜类化合物，即镰刀菌酸 A-H ()。通过光谱数据分析、量子化学计算和 X 射线晶体学解析，阐明了它们的结构。镰刀菌酸 A () 和 C-H () 是典型的异贝壳杉烯型二萜类化合物，而镰刀菌酸 B () 含有笼状结构，具有不寻常的 7,8-断异贝壳杉烯骨架。提出了的生物合成途径。在 200 μg/mL 下，进一步评估了镰刀菌酸 A () 和 C-H () 对番茄幼苗的化感作用。其中，镰刀菌酸 F () 对番茄幼苗下胚轴和根伸长的抑制作用最强，抑制率分别为 61.3%和 45.3%。